Topic
Microbacterium kyungheense
About: Microbacterium kyungheense is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 16 citations.
Papers
TL;DR: Two novel strains were characterized using a polyphasic approach to determine their taxonomic positions and 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus Microbacterium.
Abstract: Two novel strains, THG-C26T and THG-C31T, were characterized using a polyphasic approach to determine their taxonomic positions. These two isolates were aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus
Microbacterium
. The quinone systems of strains THG-C26T and THG-C31T contained MK-12/MK-13 as major menaquinones. The diamino acid in cell-wall hydrolysates of the two strains was ornithine. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polyamine pattern had spermidine as the predominant component. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. Phenotypic characteristics supported the affiliation of strains THG-C26T and THG-C31T to the genus
Microbacterium
. Chemotaxonomic data and DNA–DNA relatedness values allowed differentiation of these strains from other species of the genus
Microbacterium
with validly published names. Strains THG-C26T and THG-C31T showed highest 16S rRNA gene sequence similarities with
Microbacterium resistens
DMMZ 1710T (98.5 %) and
Microbacterium trichothecenolyticum
IFO 15077T (98.8 %), respectively, and the 16S rRNA gene sequence similarity between them was 99.0 %. DNA–DNA hybridization values between the novel isolates and strains of other species of the genus
Microbacterium
with validly published names were 4–25 %. Therefore, strains THG-C26T and THG-C31T are considered to represent two novel species of the genus
Microbacterium
, for which the names Microbacterium kyungheense sp. nov. [type strain THG-C26T ( = KACC 17124T = JCM 18735T)] and Microbacterium jejuense sp. nov. [type strain THG-C31T ( = KACC 17123T = JCM 18734T)] are proposed.
23 citations
TL;DR: A combination of DNA-DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-LLCT could be distinguished from its closely related strains.
Abstract: A Gram-positive, aerobic, non-motile, non-spore-forming, short rod-shaped strain, NEAU-LLCT, was isolated from cow dung in Shangzhi City, Heilongjiang Province, Northeast China and identified by a polyphasic taxonomic study. Colonies was light yellow, round, with entire margin. Strain NEAU-LLCT was grown at 15–45 ℃ and pH 6.0–10.0. NaCl concentration ranged from 0 to 5% (W/V). The 16S rRNA gene sequence of NEAU-LLCT showed the high similarities with Microbacterium kyungheense JCM 18735T (98.5%), Microbacterium trichothecenolyticum JCM 1358T (98.3%) and Microbacterium jejuense JCM 18734T (98.2%). The whole-cell sugars were glucose, rhamnose and ribose. The menaquinones contained MK-12 and MK-13. Ornithine, glutamic acid, lysine and a small amount of alanine and glycine were the amino acids in the hydrolyzed products of the cell wall. The major fatty acids were iso-C16:0, iso-C18:0, anteiso-C15:0 and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The genome of NEAU-LLCT was 4,369,375 bp and G + C content is 70.28 mol%. A combination of DNA–DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-LLCT could be distinguished from its closely related strains. Therefore, the strain NEAU-LLCT was considered to represent a novel species, which was named Microbacterium helvum sp. (Type strain NEAU-LLCT = CCTCC AA 2018026T = JCM 32661T).
4 citations
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2014 | 1 |