Topic
Methylococcus
About: Methylococcus is a research topic. Over the lifetime, 24 publications have been published within this topic receiving 1077 citations.
Papers
TL;DR: Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites throughout eastern Australia, and clarified group I and II methnotroph genus and species interrelationships.
Abstract: Numerical taxonomic, DNA-DNA hybridization, and phospholipid fatty acid composition analyses were performed on an extensive range of methanotrophic strains, including reference strains and environmental isolates obtained from sites throughout eastern Australia. When the results of these studies were related to the results of a study based on genomic physicochemical properties, they clarified group I and II methanotroph genus and species interrelationships. The group I methanotrophs were found to be made up of three broadly phenotypically and genotypically homologous clusters of species. The first group I methanotroph cluster included the carotenoid-containing species Methylomonas methanica, Methylomonas fodinarum, and Methylomonas aurantiaca. These species represent the true members of the genus Methylomonas. The second group I methanotroph cluster was made up of two subclusters of strains. One subcluster included species not capable of producing resting cells and consisted of the species “Methylomonas agile,” “Methylomonas alba,” and Methylomonas pelagica. The other subcluster included species capable of forming desiccation-resistant cysts and included Methylococcus luteus, marine Methylomonas-like strains, and Methylococcus whittenburyi. Strains designated “Methylococcus ucrainicus” and Methylococcus vinelandii were found to be synonyms of Methylococcus whittenburyi, while Methylococcus bovis was a synonym of Methylococcus luteus. It is proposed that these subclusters represent a new genus, Methylobacter gen. nov. The species in the new genus are type species Methylobacter luteus comb. nov., Methylobacter agilis sp. nov., Methylobacter albus sp. nov., nom. rev., Methylobacter marinus sp. nov., Methylobacter pelagicus comb. nov., and Methylobacter whittenburyi comb. nov. The remaining group I methanotrophs included the moderately thermophilic species Methylococcus capsulatus and Methylococcus thermophilus and a group of unnamed strains closely related to Methylococcus capsulatus. It is proposed that these species represent the true members of the genus Methylococcus. The group II methanotrophs consisted of two closely related groups. The first group included budding, exospore-producing strains, while the second group included nonmotile, cyst-forming strains. These groups represent the genera Methylosinus and Methyocystis, which are revived here. The genus Methylosinus gen. nov., nom. rev. includes the species Methylosinus trichosporium sp. nov., nom. rev. and Methylosinus sporium sp. nov., nom. rev., while the genus Methylocystis gen. nov., nom. rev. includes the species Methylocystis parvus sp. nov., nom. rev. and Methylocystis echinoides sp. nov., nom. rev.
445 citations
TL;DR: A 5.8 kb fragment of chromosomal DNA from Methylococcus capsulatus (Bath) containing genes encoding the soluble methane monooxygenase enzyme complex was used as a probe for the detection of soluble mono Oxygenase genes in a number of representative strains of obligate methanotrophs.
Abstract: 1. Summary A 5.8 kb fragment of chromosomal DNA from Methylococcus capsulatus (Bath) containing genes encoding the soluble methane monooxygenase enzyme complex was used as a probe for the detection of soluble monooxygenase genes in a number of representative strains of obligate methanotrophs. Only type II methanotrophs of the genus Methylosinus were found to contain homologues to the Methylococcus gene probe. This probe was also used successfully to detect soluble methane monooxygenase genes in a variety of methanotrophs by colony hybridizations.
52 citations
1 Jan 1992
43 citations
TL;DR: The accumulation of intracellular storage granules by methylococcus NCIB 11083 when grown under conditions of ammonia limitation with methane as the sole source of carbon and energy was inversely proportional to the dilution rate.
Abstract: The accumulation of intracellular storage granules (003--05 micrometer) by Methylococcus NCIB 11083 when grown under conditions of ammonia limitation with methane as the sole source of carbon and energy was inversely proportional to the dilution rate The isolated material was composed entirely of glucose residues and the infra-red spectrum exhibited characteristic absorption bands at 925 cm(-1), 845 cm(-1) and 745 +/- 4cm(-1), indicating the presence of alpha (1 leads to 4) glycosidic linkages The polymer dissolved in hot water to give an opalescent solution that formed a violet iodine complex with an absorption maximum at 550nm, identical to that observed with reference amylopectin The percentage of the polysaccharide released as maltose by the action of beta- and alpha-amylases was 55--64% and 80--90% respectively, values very similar to those obtained by the action of these enzymes on reference amylopectin and glycogen Methylation analysis indicated that the average interior and exterior chain lengths of the polymer were 27 and 100 glucose units respectively and confirmed that the Methylococcus polyglucose is a branched polymer composed of units joined by 1 leads to 4 and 1 leads to 6 linkages The number average molecular weight of the polymer is 2--45 x 10(5) The stored polymer was metabolised by the organism and its metabolism resulted in the synthesis of protein
38 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
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| 2021 | 2 |
| 2020 | 2 |
| 2019 | 2 |
| 2011 | 1 |
| 2000 | 1 |
| 1998 | 1 |