Topic
Methylobacterium iners
About: Methylobacterium iners is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 120 citations.
Papers
TL;DR: Two bacterial strains isolated from air samples were characterized by determining their phenotypic characteristics, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis, and it was concluded that strains 5317S-33(T) and 5413S-11 (T) represent two novel species within the genus Methylobacterium.
Abstract: Two bacterial strains isolated from air samples, 5317S-33T and 5413S-11T, were characterized by determining their phenotypic characteristics, cellular fatty acid profiles and phylogenetic positions based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that these isolates belonged to the genus Methylobacterium. Strain 5317S-33T was most closely related to Methylobacterium adhaesivum AR27T (97.9 % sequence similarity). Strain 5413S-11T was most closely related to Methylobacterium fujisawaense DSM 5686T (97.3 % sequence similarity), Methylobacterium oryzae CBMB20T (97.1 % similarity) and Methylobacterium radiotolerans JCM 2831T (97.0 % similarity). Cells of both strains were strictly aerobic, Gram-negative, motile and rod-shaped. The major fatty acid was C18 : 1
ω7c. The G+C contents of the genomic DNA were 68.0 mol% for strain 5317S-33T and 73.2 mol% for strain 5413S-11T. According to DNA–DNA hybridization data, strain 5317S-33T showed a level of DNA–DNA relatedness of 33 % with M. adhaesivum DSM 17169T, and strain 5413S-11T showed low levels of DNA–DNA relatedness (<35 %) with M. fujisawaense DSM 5686T, M. oryzae CBMB20T and M. radiotolerans DSM 1819T. On the basis of this polyphasic analysis, it was concluded that strains 5317S-33T and 5413S-11T represent two novel species within the genus Methylobacterium, for which the names Methylobacterium iners sp. nov. (type strain 5317S-33T =KACC 11765T =DSM 19015T) and Methylobacterium
aerolatum sp. nov. (type strain 5413S-11T =KACC 11766T =DSM 19013T) are proposed.
TL;DR: It is proposed that strain Gh-105(T) represents the type strain of a novel species, with the name Methylobacterium gossipiicola sp.
Abstract: A pink, aerobic, facultatively methylotrophic, motile, Gram-negative rod, designated Gh-105(T), was isolated from the phyllosphere of cotton from Coimbatore (Tamilnadu, India). 16S rRNA gene sequence analysis showed clearly that the isolate belonged to the Methylobacterium cluster. Strain Gh-105(T) was most closely related to Methylobacterium adhaesivum AR27(T) (99% 16S rRNA gene sequence similarity) and Methylobacterium iners 5317S-33(T) (97.5%). The isolate grew with C(1) compounds such as methanol and dichloromethane, but not with formaldehyde, formate, methylamine, trimethylamine or methane, as sole carbon sources and carried mxaF, which encodes methanol dehydrogenase and supports methylotrophic metabolism. The major fatty acid was C(18:1)ω7c and the G+C content of the genomic DNA was 64.2 mol%. Physiological and biochemical data and DNA-DNA relatedness with M. adhaesivum KACC 12195(T) and M. iners KACC 11765(T) revealed clear phenotypic and genotypic differences. For this reason, we propose that strain Gh-105(T) (=CCM 7572(T) =NRRL B-51692(T)) represents the type strain of a novel species, with the name Methylobacterium gossipiicola sp. nov.
TL;DR: The results of DNA-DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35a(T) from its nearest phylogenetic neighbours.
Abstract: A pink-pigmented, facultatively methylotrophic bacterium, strain 35a(T), was isolated from the leaves of Oxalis corniculata. Cells of strain 35a(T) were Gram-reaction-negative, motile, non-spore-forming rods. The highest 16S rRNA gene pairwise sequence similarities for strain 35a(T) were found with the strains of Methylobacterium iners 5317S-33(T) (96.7%), 'Methylobacterium soli' YIM 48816 (96.6%) and Methylobacterium jeotgali S2R03-9(T) (96.3%). 16S rRNA gene sequence similarities with the type strains of all other recognized species of the genus Methylobacterium were below 96%. Major cellular fatty acids were C(18:1)ω7c, C(18:0) and C(16:0). The results of DNA-DNA hybridization experiments, analysis of cpn60 gene sequences, fatty acid profiles, whole-cell MALDI-TOF/MS spectral pattern analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 35a(T) from its nearest phylogenetic neighbours. Strain 35a(T) is therefore considered to represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oxalidis sp. nov. is proposed. The type strain is 35a(T) (=DSM 24028(T)=NBRC 107715(T)).
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2012 | 2 |
| 2008 | 1 |