TL;DR: A rod-shaped extremely thermophilic methanogen is described, growing between 65 and 97 °C with an optimal temperature around 83 °C and a doubling time of 170 min, indicating that the isolate belongs to a new family, the Methanothermaceae, within the order Methanobacteriales.
TL;DR: The predominant cell wall polymer of Methanobacteriales is pseudomurein, which distinguishes this order from the Methanomicrobiales, which is generally hydrogenotrophic, using H2 to reduce CO2 to CH4.
Abstract: Members of the order Methanobacteriales are distinguished from other methanogens by their limited range of catabolic substrates, their morphology, lipid composition, and rDNA sequence. The Methanobacteriales are generally hydrogenotrophic, using H2 to reduce CO2 to CH4. Some members of this order can use formate, CO, or secondary alcohols as electron donors for CO2 reduction. However, members of one genus within this order, Methanosphaera, use H2 to reduce methanol to methane. The predominant cell wall polymer of Methanobacteriales is pseudomurein, which distinguishes this order from the Methanomicrobiales. Lipids composing the cell membranes include caldarchaeol and myo-inositol. Cells usually stain Gram positive and are generally rod-shaped, often forming chains or long filaments up to 40 μm in length. The order Methanobacteriales is divided into two families, the Methanobacteriaceae and Methanothermaceae, on the basis of phylogenetic analysis of 16S rRNA gene sequences as well as phenotypic characteristics (Boone, 2001).
TL;DR: The predominant cell wall polymer of Methanobacteriales is pseudomurein, which distinguishes this order from the Methanomicrobiales, which is generally hydro- genotrophic, using H2 to reduce CO2 to CH4.
Abstract: Members of the order Methanobacteriales are distinguished from other methanogens by their limited range of catabolic substrates, their mor- phology, lipid composition, and rDNA sequence. The Methanobacteriales are generally hydro- genotrophic, using H2 to reduce CO2 to CH4. Some members of this order can use formate, CO, or secondary alcohols as electron donors for CO2 reduction. However, members of one genus within this order, Methanosphaera ,u se H2 to reduce methanol to methane. The predominant cell wall polymer of Methanobacteriales is pseudomurein, which distinguishes this order from the Methanomicrobiales. Lipids composing the cell membranes include caldarchaeol and myo-inositol. Cells usually stain Gram positive and are generally rod-shaped, often forming chains or long filaments up to 40 µm in length. The order Methanobacteriales is divided into two families, the Methanobacteriaceae and Methanothermaceae, on the basis of phyloge- netic analysis of 16S rRNA gene sequences as well as phenotypic characteristics (Boone, 2001).