TL;DR: In this paper, detailed GC and GC-MS analyses of oil of Melaleuca alternifolia have identified several constituents not previously reported and clarified some earlier assignments, and the range, mean and variance of variation for the principle constituents in 800 typical samples were presented along with the compositions of several substandard oils.
Abstract: Detailed GC and GC-MS analyses of oil of Melaleuca have identified several constituents not previously
reported from Melaleuca alternifolia and clarified some earlier assignments. The range, mean, and
coefficient of variation for the principle constituents in 800 typical samples are presented along with
the compositions of several substandard oils. Isolation and storage procedures affecting the chemical
composition of the oil are reported. Ethanolic extraction of mature leaves gave solutions suitable for
direct injection into a gas chromatograph for the qualitative determination of tea tree oil. Comparison
with conventional steam distillation showed that this technique was suitable for preliminary analysis
of tea tree oil yield and quality.
TL;DR: Essential oil of Melaleuca alternifolia was tested for antiviral activity against Tobacco Mosaic Virus and was effective in significantly decreasing lesion numbers for at least 10 days post inoculation.
Abstract: Essential oil of Melaleuca alternifolia was tested for antiviral activity against Tobacco Mosaic Virus When applied to plants of Nicotiana glutinosa as a pre-inoculation spray at 100, 250 and 500 ppm, the oil was effective in significantly decreasing lesion numbers for at least 10 days post inoculation
TL;DR: Differences were shown between the three essential oils in their action on smooth muscle: Manuka had a spasmolytic action, while Kanuka and Melaleuca had an initial spasmogenic action and the variability in the Manuka and Kanuka essential oils suggests caution in their usage, as does the fact that the oils have not been tested for toxicity.
Abstract: Three different species of Myrtaceae growing in Australia and New Zealand are known as 'Tea-tree': the Australian Tea tree (Melaleuca alternifolia), the New Zealand Manuka (Leptospermum scoparium) and Kanuka (Kunzea ericoides). All three essential oils are used by aromatherapists, although only Melaleuca has been tested for toxicity, and its antimicrobial effects studied. The pharmacology and antimicrobial activity of the three 'tea-tree' oils was determined using guinea-pig ileum, skeletal muscle (chick biventer muscle and the rat phrenic nerve diaphragm) and also rat uterus in vitro. Differences were shown between the three essential oils in their action on smooth muscle: Manuka had a spasmolytic action, while Kanuka and Melaleuca had an initial spasmogenic action. Using the diaphragm, Manuka and Melaleuca decreased the tension and caused a delayed contracture; Kanuka had no activity at the same concentration. The action on chick biventer muscle was, however, similar for all three oils, as was the action on the uterus, where they caused a decrease in the force of the spontaneous contractions. The latter action suggests caution in the use of these essential oils during childbirth, as cessation of contractions could put the baby, and mother, at risk. The comparative antimicrobial activity showed greater differences between different samples of Manuka and Kanuka than Melaleuca samples. The antifungal activity of Kanuka was inversely proportional to its strong antibacterial activity, whilst Manuka displayed a stronger antifungal effect, though not as potent as Melaleuca. The antioxidant activity of Manuka samples was more consistent than that of Kanuka, while Melaleuca showed no activity. The variability in the Manuka and Kanuka essential oils suggests caution in their usage, as does the fact that the oils have not been tested for toxicity.
TL;DR: In vitro antifungal activity of the essential oils from Monarda citriodora and Melaleuca alternifolia was evaluated on 15 common post-harvest pathogens of a variety of crops, particularly against more rapidly growing fungal species.
Abstract: In vitro antifungal activity of the essential oils from Monarda citriodora and Melaleuca alternifolia was evaluated on 15 common post-harvest pathogens of a variety of crops. Both oils exhibited a high level of antifungal activity both by direct contact and in the vapor phase. Oil from Monarda citriodora was generally more active than that from Melaleuca alternifolia, particularly against more rapidly growing fungal species.