Topic
Mannosamine
About: Mannosamine is a research topic. Over the lifetime, 236 publications have been published within this topic receiving 5902 citations. The topic is also known as: D-ManN & Manosamine.
Papers published on a yearly basis
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Papers
TL;DR: In this paper, a method was developed to determine the aldononitrile acetate derivatives of the four amino sugars simultaneously in the 6 m HCl hydrolysates of soil samples by means of high resolution gas liquid chromatography.
Abstract: Muramic acid, glucosamine, mannosamine and galactosamine in soils may be useful for elucidating the microbial origin of soil organic nitrogen. Therefore, a method was developed to determine the aldononitrile acetate derivatives of the four amino sugars simultaneously in the 6 m HCl hydrolysates of soil samples by means of high resolution gas liquid chromatography. This method was sensitive enough to detect less than 10 μg muramic acid ml−1 and less than 20 μg ml−1 of the other three amino sugars. The maximum release of amino sugars was found after 6 to 8 h hydrolysis at 105°C. Impurities in the acidic hydrolysates were removed simply by neutralisation with KOH solution. The recovery of amino sugars after hydrolysis and purification was more than 90% on average. The method was applied to determine amino sugars in eight soils with different properties. The coefficients of variation averaged 6.1% for glucosamine and galactosamine and 10.9% for muramic acid and mannosamine.
642 citations
TL;DR: It is shown that in rat the normally occurring N-acetyl neuraminic acid can be modified in its N-acyl moiety by in vivo administration of the chemically synthesized N-propanoyl precursors, N- ProPanoyl-D-glucosamine or N-Propanoy l-mannosamine, which may provide a tool for a further characterization of the biological function of sialic acids.
299 citations
TL;DR: In this paper, a reverse-phase high-performance liquid chromatography method was improved for the simultaneous determination of muramic acid, mannosamine, glucosamine and galactosamine in soil and plant hydrolysates via ortho-phthaldialdehyde (OPA) pre-column derivatisation and fluorescence detection.
Abstract: Amino sugars are increasingly used as indicators for the accumulation of microbial residues in soil and plant material. A reverse-phase high-performance liquid chromatography method was improved for the simultaneous determination of muramic acid, mannosamine, glucosamine and galactosamine in soil and plant hydrolysates via ortho-phthaldialdehyde (OPA) pre-column derivatisation and fluorescence detection. The retention time was reduced, and the separation of muramic acid and mannosamine was optimised by modifying the mobile phase. The effects of excitation wavelength, OPA reaction time, tetrahydrofuran concentration and pH value of the mobile phase on the amino sugar separation were tested. Quantification limits were in the range of 0.13 to 0.90 μg ml−1. No interferences exist from amino acids or other primary amines, occurring in soil and plant hydrolysates.
146 citations
TL;DR: Group A meningococcal polysaccharide was shown to be a homopolymer of d-mannosamine phosphate which is partially N- and O-acetylated and there is evidence to suggest that other glycosidic linkages exist.
143 citations
TL;DR: Throbin-activated human platelets cause agglutination of trypsinized, formalinized bovine erythrocytes and it appears that a membrane surface component that has lectin activity mediates platelet aggregation.
Abstract: Throbin-activated human platelets cause agglutination of trypsinized, formalinized bovine erythrocytes. This lectin activity of stimulated platelets was blocked by galactosamine, glucosamine, mannosamine, lysine, and arginine, but not by N-acetylated sugars, other neutral sugars, or other amino acids. Inhibitors of the thrombin-induced lectin activity also blocked thrombin-induced platelet aggregation. It appears that a membrane surface component that has lectin activity mediates platelet aggregation.
117 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2020 | 3 |
| 2019 | 3 |
| 2017 | 1 |
| 2016 | 3 |
| 2015 | 5 |