TL;DR: In this article, the impacts of different concentrations (0, 10, 100, and 500 mg/l−1) of nanosized (10−25nm) titanium dioxide (TiO2) on growth, seed yield, photosynthetic pigment contents, the values of hydrogen peroxide (H2O2), and malondialdehyde (MDA), seed oil, and protein contents in Linum usitatissimum Linea (Linaceae) under sufficient and scarce water conditions.
Abstract: Drought stress has detrimental effects on growth and yield of plants under arid or semi-arid environment. Because of their potential for modulating the redox status and changing the growth, performance and quality of plants, nano-scale materials are among the research interests of physiologists. This study evaluated the impacts of different concentrations (0, 10, 100, and 500 mg l−1) of nanosized (10–25 nm) titanium dioxide (TiO2) on growth, seed yield, photosynthetic pigment contents, the values of hydrogen peroxide (H2O2) and malondialdehyde (MDA), seed oil, and protein contents in Linum usitatissimum Linea (Linaceae) under sufficient and scarce water conditions. The results showed that application of nanoscale TiO2 at low concentration better improved the morphological and physiological traits of plant compared to other doses particularly under water scare conditions, leading to better plant performance. Enhanced chlorophyll and carotenoids contents were recorded in leaves of nano-anatase TiO2-treated plants under both normal and drought stress conditions, when compared to the control. The levels of H2O2 and MDA in plants exposed to nano TiO2 at 10 mgl−1 were lower than that of other treatments, therefore, lipid peroxidation was less pronounced in such plants. In both well watered and drought stress conditions, the highest values of seed oil and protein contents were obtained in plants treated with nano TiO2 at 100 mg l−1. Therefore, exogenous application of nano TiO2 particles at appropriate concentrations can ameliorate drought stress damage to Flax seed plants as well as increase the drought tolerance with remarkable improvement in physiological process.
TL;DR: It is interesting is that the transgenic plants showed a significant decrease in lignin content, which may lead to the improvement of flax fibre retting.
TL;DR: A significant increase in monounsaturated fatty acids and a slight increase in lignans content accompanied the increase in antioxidant properties of flaxseeds, indicating a strong relationship between phenolic acids content and antioxidant capacity.
Abstract: The principal goal of this paper was to generate flax (Linum usitatissimum L.) plants with increased antioxidant properties. To accomplish this a vector containing a multigene construct was prepared, and transgenic plants overexpressing essential flavonoid biosynthesis pathway enzymes were generated and analyzed. The simultaneous expression of genes encoding chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR) resulted in a significant increase of flax antioxidant capacity. To investigate the determinants of higher antioxidant properties of transgenic plants, the phenolic acids and lignans compound contents were measured. In both green part and seed extracts from transgenic plants, the phenolic acids level was increased when compared to the control. The calculated correlation coefficient between phenolic acids content and antioxidant capacity (0.82 and 0.70 for green part and flaxseed, respectively) perfectly reflects their strong relationship. The increase in yield of transgenic plants and their higher resistance to Fusarium culmorum and Fusarium oxysporum when compared to the control plants was a characteristic feature. It was assessed a very high correlation (correlation coefficient = 0.9) between phenolic acids level in flaxseed extract and resistance to F. culmorum. The flowering date of transgenic plants was approximately 3 weeks earlier than that of the control plants. Interestingly, a significant increase in monounsaturated fatty acids and a slight increase in lignans content accompanied the increase in antioxidant properties of flaxseeds.
TL;DR: A phytochemical study of Linum album (Linaceae), guided by bioassay with the 9KB cell culture, resulted in the isolation of podophyllotoxin and a new lignan, 3'-demethylpodophyLLotoxin; alpha- and beta-peltatins were identified by comparative TLC.