Topic
Lilium regale
About: Lilium regale is a research topic. Over the lifetime, 89 publications have been published within this topic receiving 581 citations.
Papers published on a yearly basis
Papers
TL;DR: The results here indicate that R2R3-MYB genes exhibiting similar (but not identical) functions are conserved between monocots and eudicots and that transcriptional regulation is a major mechanism for generating restricted pigment deposition in the flowers of a wide range of angiosperm species.
Abstract: Color patterns in angiosperm flowers are produced by spatially and temporally restricted deposition of pigments. Many Lilium species show anthocyanin pigmentation of the whole tepal, which is regulated by the MYB12/basic helix-loop-helix 2 (bHLH2) transcription factor complex. However, in Lilium regale pigment deposition is restricted to light-exposed surfaces of the flower buds, leaves, and bracts. Investigation of how the unique color patterns of L. regale are regulated led to the identification and isolation of a novel R2R3-MYB gene, LrMYB15. In stable and transient transformation assays in tobacco, LrMYB15 stimulated transcription of anthocyanin biosynthesis genes, indicating that it is involved in the positive regulation of anthocyanins. In L. regale, anthocyanin biosynthesis genes and LrMYB15 were expressed in the outer tepals, leaves, and bracts that accumulated anthocyanin pigments. In contrast, LrbHLH2, another regulatory gene for anthocyanin biosynthesis, was transcribed in all organs irrespective of anthocyanin accumulation. These results indicate that LrMYB15 principally determines the unique anthocyanin color patterns of L. regale. In addition, LrMYB15 transcription ceased completely when plants were kept in shaded conditions and the colors of the flower buds faded, indicating that transcription of this gene is under the control of light. R2R3-MYB genes that regulate light-induced anthocyanin accumulation on exposed petal surfaces have been isolated in eudicots. The results here indicate that R2R3-MYB genes exhibiting similar (but not identical) functions are conserved between monocots and eudicots and that transcriptional regulation is a major mechanism for generating restricted pigment deposition in the flowers of a wide range of angiosperm species.
76 citations
TL;DR: The informative EST-SSR markers derived from expressed sequence tags (EST-SSRs) based on transcriptome sequences in Lilium regale will be very useful in subsequent Lilium genetic improvement projects.
Abstract: Simple-sequence repeat (SSR) markers are a common choice for assaying genetic diversity and genetic mapping due to their highly polymorphic and co-dominant nature. In this paper, we developed SSR markers derived from expressed sequence tags (EST-SSRs) based on transcriptome sequences in Lilium regale. In total, 59,046 UniGene sequences (approximately 27.37 Mb) were evaluated, where 1,716 SSR sites in the 1,606 UniGene sequences were identified by data mining. Of these SSRs, the trinucleotide repeats were the most common repeat types (631, 36.8 %), followed by hexa- (23.7 %), di- (23.4 %), penta- (11.5 %), tetra- (3.3 %), hepta- (0.9 %), deca- (0.3 %), and octanucleotide repeats (0.1 %). TC/GA and CCG/CGG predominated in dimeric and trimeric repeat motifs, respectively. In total, 1,072 primer pairs were designed, of which 494 were amplified to yield the expected PCR products in L. regale. The polymorphism and transferability study was performed in six species endemic to China and ten cultivars derived from intraspecific and interspecific hybridization. Of the 494 primer pairs, 172 exhibited clear polymorphisms with stable cross-species amplifications in the 16 individuals, which contained 537 alleles for the 181 loci. The polymorphism information content values for the 172 primers ranged from 0.111 to 0.830, with an average value of 0.493. The phylogenetic dendrogram derived from the amplification profiles of the 172 polymorphic primers was congruent with the genetic background of the hybrids and the species. In addition, the putative functions of the 109 UniGenes containing the 118 polymorphic and transferable EST-SSRs were identified. Thus, the informative EST-SSR markers will be very useful in subsequent Lilium genetic improvement projects.
44 citations
TL;DR: A positive role of transcriptome‐derived LrNAC35 in transcriptional modulation of host defence against viral attack is suggested.
Abstract: Cucumber mosaic virus (CMV) is a highly prevalent viral pathogen causing substantial damage to the bulb and cut-flower production of Lilium spp. Here, we performed an Illumina RNA sequencing (RNA-Seq) study on the leaf tissues of a virus-resistant species Lilium regale inoculated with mock control and CMV. A total of 1346 differentially expressed genes (DEGs) were identified in the leaves of L. regale upon CMV inoculation, which contained 34 up-regulated and 40 down-regulated DEGs that encode putative transcription factors (TFs). One up-regulated TF, LrNAC35, belonging to the NAM/ATAF/CUC (NAC) superfamily, was selected for further functional characterization. Aside from CMV, lily mottle virus and lily symptomless virus infections provoked a striking increase in LrNAC35 transcripts in both resistant and susceptible Lilium species. The treatments with low temperature and several stress-related hormones activated LrNAC35 expression, contrary to its reduced expression under salt stress. Ectopic overexpression of LrNAC35 in petunia (Petunia hybrida) resulted in reduced susceptibility to CMV and Tobacco mosaic virus infections, and enhanced accumulation of lignin in the cell walls. Four lignin biosynthetic genes, including PhC4H, Ph4CL, PhHCT and PhCCR, were found to be up-regulated in CMV-infected petunia lines overexpressing LrNAC35. In vivo promoter-binding tests showed that LrNAC35 specifically regulated the expression of Ph4CL. Taken together, our results suggest a positive role of transcriptome-derived LrNAC35 in transcriptional modulation of host defence against viral attack.
42 citations
TL;DR: This study provides valuable information about the structural and functional characterization of LrWRKY genes that will not only deepen the understanding of the molecular mechanisms underlying the defense of lily against B. cinerea but also offer potential targets for cultivar improvement via biotechnology.
39 citations
TL;DR: Two new and three known steroidal Saponins were isolated from the fresh bulbs of Lilium regale and two known saponins from those of L. henryi and their inhibitory activity on cyclic AMP phosphodiesterase was evaluated.
38 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 5 |
| 2020 | 5 |
| 2019 | 10 |
| 2018 | 5 |
| 2017 | 3 |
| 2016 | 7 |