TL;DR: Molecular data suggest that the remaining genera of Massonieae do not share common ancestry with the Eurasian/North-African tribe Hyacintheae Dumort, and thus a narrow concept of the essentially Eurasian genus Scilla is supported.
Abstract: The tribe Massonieae Baker (Hyacinthaceae-Hyacinthoideae) presently consists of about 19 genera and 230 species distributed from Africa (south of the Sahara) to Madagascar and India. Based on atpB and trnL-F DNA sequences the tribe is monophyletic only when the genus Pseudoprospero is excluded from Massonieae. In most trnL-F trees, this genus occupies a basal position within subfamily Hyacinthoideae and is sister to the rest of the subfamily. Molecular data suggest that the remaining genera of Massonieae do not share common ancestry with the Eurasian/North-African tribe Hyacintheae Dumort. (Scilla, Hyacinthus and allies), and thus a narrow concept of the essentially Eurasian genus Scilla is supported. Members of well-supported clades in Massonieae usually show similarities in seed characteristics as determined by scanning electron microscopy. Phylogenetic position and seed morphology indicate that Massonia angustifolia and M. zeyheri do not belong to the genus Massonia but fall into a clade together with Daubenya, Androsiphon and Amphisiphon. The genus Whiteheadia appears paraphyletic in the 50% majority rule trnL-F tree and occupies a basal position next to Massonia. However, in the strict consensus tree neither monophyly nor polyphyly can be excluded for this genus. Seed appendages are documented for members of the genera Ledebouria and Lachenalia. Within the genera of Massonieae there is a tendency towards bending of the seed axis. This phenomenon is most obvious within the genus Lachenalia. Delimitation of genera based on seed morphology largely agrees with the results of molecular studies. Correlation between number, size and color of seeds, geographical distribution and phylogenetic position of the genera are discussed.
TL;DR: Bulbine species yielded novel phenyl anthraquinones and isofuranonaphthoquinones, which were found to exhibit antiparasitic and antioxidant properties, and some of the homoisoflavonoids were finding to possess activity against colon and breast cancer cell lines.
Abstract: The sale of non-cultivated plants poses a real threat to the survival of a large number of plant species. On the other hand, the fact that a given plant has become a traded item is an important lead in the effort to investigate plants for the purpose of discovering novel secondary metabolites that may have marketable properties. This report is largely based on the investigations of plants collected from vendors of medicinal plants. The identification is based on botanical specimens derived from the purchased plant parts either by growing them in a garden or through micropropagation using tissue culture techniques. Bulbine species yielded novel phenyl anthraquinones and isofuranonaphthoquinones, which were found to exhibit antiparasitic and antioxidant properties. Scilla nervosa subsp. rigidifolia and Ledebouria graminifolia yielded twenty homoisoflavonoids and two xanthones. Bulbs derived from tissue culture based specimens of L. graminifolia contained all the compounds isolated from the marketed samples. Some of the homoisoflavonoids were found to possess activity against colon and breast cancer cell lines. Rhus pyroides furnished six novel bichalcones some of which exhibited insect antifeedant, cytotoxic and antiproliferative activities. Structures of the compounds described in this report were elucidated by spectroscopic means including HRMS and 1D-, and 2D-NMR experiments. Interconversion of some of the phenyl anthraquinones was accomplished as part of their structure proof. The total synthesis of three of the biflavonoids was achieved using a synthetic route which involves a microwave irradiation promoted Ulmann synthesis.
TL;DR: Supporting evidence is found that U. volubilis is not a member of the subfamily Urgineoideae but belongs to theSubfamily Ornithogaloideae with strong affinity to the Stellarioides/Albuca/Coilonox clade.
Abstract: In this paper we report on three endemic Hyacinthaceae of Madagascar originally described by PERRIER 1935 as Ornithogalum convallarioides, Scilla nossibeensis and Urginea volubilis (Fig. 3). TrnL-F data of these three species are provided for the first time. Based on these data we suggest to include S. nossibeensis in the genus Ledebouria (as already suggested by MANNING & al. 2004) of the subfamily Hyacinthoideae, whereas the other two taxa belong to the subfamily Ornithogaloideae. O. convallarioide is best treated as member of the genus Avonsera as suggested by SPETA 1998a, b. Contrary to previous examinations we have supporting evidence that U. volubilis is not a member of the subfamily Urgineoideae but belongs to the subfamily Ornithogaloideae with strong affinity to the Stellarioides/Albuca/Coilonox clade. Since genus boundaries in this group are not yet fixed we follow for the time being the suggestion of SPETA 1998a who combined this taxon to Igidia volubilis (PERRIER) SPETA. We found the diploid chromosome numbers 2n=14 for Avonsera convallarioides, 2n=12 for Igidia volubilis and 2n=30 for Ledebouria nossibeensis. In A. convallarioides we observed protein crystals in the nuclei, but we were not able to find such crystals in the other two species.