TL;DR: Among the major oil palm pest insects in the Côte d'Ivoire,atoia viridissima Holland is the most frequently observed defoliator and a natural epizootic permitted us to demonstrate the occurrence of a small isometric RNA virus of 30nm in diameter.
Abstract: Among the major oil palm pest insects in the Cote d'Ivoire,Latoia viridissima Holland [Lepidoptera, Limacodidae] is the most frequently observed defoliator. During a pullulation of this species, a natural epizootic permitted us to demonstrate the occurrence of a small isometric RNA virus of 30nm in diameter. The buoyant density of the virus particles was 1.34. The virus capsid contained 2 major proteins with molecular weights of 30,000 (55%) and 31,000 (20%) and 3 minor proteins. One genome component was detected with molecular weight 2,9×106. Agarose gel diffusion tests showed this virus was distinct from any other described insect Picornavirus. Trials with different doses of viral suspensions were tested on industrial oil palm plantation, allocated byL. viridissima, from ground level, using an automatic air carried sprayer. One week after the treatment, a mortality gradient, increasing from 11 to 61% according to the dose applied, was obtained. Two weeks after the treatment the mortality reached 92% of the larvae in the treated parcels. During the next generation, the number of caterpillars on the same parcel was very low.
TL;DR: Data from cocoon samples showed that the sexes of the moth could be separated on the basis of cocoon size, and major mortality factors were an aggregate of natural enemies, particularly predators, parasitoids and pathogens responsible for 60–80% mortality throughout the entire larval life.
Abstract: Techniques for assessing infestation trends of Latoia viridissima are presented in this report. A plot of the head capsule widths of larvae indicated six larval instars with instars one and two being more abundant. It also illustrated the composition of larval instars in the population and hence would serve as a standard against which future samples of the species could be compared. Larval survivorship data indicated heavy mortality (>60%) during instars one and two. Major mortality factors were an aggregate of natural enemies, particularly predators (causing 40–50% of the death during the age interval), parasitoids and pathogens responsible for 60–80% mortality throughout the entire larval life. The residual cocoon populations were further reduced by about 20–30% by parasitoids (Brachymeria sp., Chrysis spina, Cocygodes coccyx, Palexorista sp and Systropus pelopoeus) and a pathogenic fungus. Data from cocoon samples showed that the sexes of the moth could be separated on the basis of cocoon size. Cocoon weights were therefore, a better criterion than cocoon diameter values for separating the sexes; since overlap between the sexes was less than 5 % for cocoon weight as against 20% In the case of cocoon diameters. The separation point tended to lie between cocoons weighing 350–399 mg. Fecundity of the females was highly correlated with female weights (r = 0.901) and wing length (r = 0.89), The calculated fecundity of 143 eggs/female (using wing length) was not statistically different (P ≤ 0.05) from the observed value of 149 eggs/female. The significance of the findings as regards forecast of outbreaks and assessment of outbreak situations are discussed.
TL;DR: The populations of L. viridissima were stable on the two palms and the key factor, i.e., the mortality of the early instar larvae, operated in the two plots, in relation to the stability of the habitats.