Topic
Laribacter
About: Laribacter is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 9 citations.
Papers
TL;DR: Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03(T) forms a distinct lineage with respect to closely related genera within the family Neisseriaceae of the class Betaproteobacteria, most closely related to the genera Aquaspirillum, Laribacter, Leeia and Microvirgula.
Abstract: A bacterial strain, designated Npb-03T, was isolated from a freshwater river in Taiwan and was characterized using a polyphasic taxonomic approach. The cells were Gram-reaction-negative, straight rod-shaped, non-motile, non-spore-forming and facultatively anaerobic. Growth occurred at 10–37 °C (optimum, 30–35 °C), at pH 6.0–8.0 (optimum, pH 6.0–7.0) and with 0–1.0 % NaCl (optimum, 0 %). The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 64.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and three uncharacterized phospholipids. The major polyamines were putrescine, 2-hydroxyputrescine, cadaverine and spermidine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-03T forms a distinct lineage with respect to closely related genera within the family
Neisseriaceae
of the class
Betaproteobacteria
, most closely related to the genera
Aquaspirillum
,
Laribacter
,
Leeia
and
Microvirgula
, and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 93 %. On the basis of the genotypic and phenotypic data, strain Npb-03T represents a novel genus and species of the family
Neisseriaceae
, for which the name Rivicola pingtungensis gen. nov., sp. nov. is proposed. The type strain is Npb-03T ( = BCRC 80376T = LMG 26668T = KCTC 23712T).
10 citations
Patent•
4 Jul 2012
TL;DR: In this paper, a method for detecting laribacter hongkongens from excrement of freshwater fish was proposed, which is based on the detection of laribacteria from the MacConkey culture medium.
Abstract: The invention provides a method for detecting laribacter hongkongensis from excrement of freshwater fish. The method comprises the following steps that: firstly, the excrement is cultured by the MacCoThe invention provides a method for detecting laribacter hongkongensis from excrement of freshwater fish. The method comprises the following steps that: firstly, the excrement is cultured by the MacConkey culture medium, and micro colonies which are colorless, semi-transparent and flat and of which the diameter is between 0.5 and 1.0mm are picked up and purely cultured; secondly, by a urease testnkey culture medium, and micro colonies which are colorless, semi-transparent and flat and of which the diameter is between 0.5 and 1.0mm are picked up and purely cultured; secondly, by a urease testand an oxidase and catalase test, the bacteria suspected to be laribacter hongkongensis are screened out preliminarily; thirdly, the screened-out bacteria are further identified by the PCR method; andand an oxidase and catalase test, the bacteria suspected to be laribacter hongkongensis are screened out preliminarily; thirdly, the screened-out bacteria are further identified by the PCR method; and finally, the laribacter hongkongens is confirmed by the Gram staining method. Primers used in the PCR method are 5'-ATCCCTAAGGCTAATACCCT-3' and 5'-AATCTCTTCGAGGTTCGG TA-3'.finally, the laribacter hongkongens is confirmed by the Gram staining method. Primers used in the PCR method are 5'-ATCCCTAAGGCTAATACCCT-3' and 5'-AATCTCTTCGAGGTTCGG TA-3'.
1 citations
Patent•
22 Jul 2015
TL;DR: A real-time fluorescent PCR detection kit for laribacter hongkonggensis and an application thereof is described in this article, where the kit includes a PCR amplification reaction liquid, a positive control and a negative control.
Abstract: The invention discloses a real-time fluorescent PCR detection kit for laribacter hongkonggensis and an application thereof, wherein the kit includes a PCR amplification reaction liquid, a positive control and a negative control; the PCR amplification reaction liquid includes a specific upstream primer, a specific downstream primer, a fluorescent probe, a PCR buffer liquid, a deoxynucleoside triphosphate mixture and DNA polymerase; the target bacteria detection sensitivity is high, the specificity is good, and the false positive rate of conventional PCR amplification can be reduced; and the kit can rapidly, accurately and specifically detect laribacter hongkonggensis.
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2015 | 1 |
| 2014 | 1 |
| 2012 | 1 |