TL;DR: The preliminary conclusion is that the animal samples are suspected for the presence of tetracycline, macrolide, and β-lactam antibiotics.
Abstract: The study compares two existing microbiological inhibition tests, Screening Test for Antibiotic Residues (STAR) and Premi ® Test with two recently introduced tests, Nouws Antibiotic Test (NAT) and Total Antibiotics for the screening of antimicrobial residues in the tissues of food-producing animals. In the negative or positive sample classification based on inhibition of the growth of test strain sensitive to many antibiotics and sulphonamides, out of 142 samples obtained from slaughterhouses and retail operations, 39 samples yielded a positive result in one or more tests: 4 samples in four tests, 14 samples in three tests, 13 samples in two tests, and 8 samples in one test. As for the numbers of observed positive samples, the descending sequence of tests was: STAR, Total Antibiotics, Premi ® Test, NAT. The growth inhibition was observed in three out of seven test strains, namely Bacillus cereus ATCC 11778, Kocuria rhizophila ATCC 9341, and Bacillus stearothermophilus var. calidolactis . Considering the test strains sensitivity and no inhibition on the Bacillus pumilus NCIMB 10822 NAT test plates, our preliminary conclusion is that the animal samples are suspected for the presence of tetracycline, macrolide, and b-lactam antibiotics.
TL;DR: The aim of this research was to study on the identification and antimicrobial activity of Streptomyces strains which were isolated from thirteen soil samples collected around Angthong Islands National Park, Surat Thani province, the southern part of Thailand.
Abstract: Streptomyces strains are superior to other actinomycete strains in their ability to produce large numbers and varieties of antibiotics. The aim of this research was to study on the identification and antimicrobial activity of Streptomyces strains which were isolated from thirteen soil samples collected around Angthong Islands National Park, Surat Thani province, the southern part of Thailand. Twenty-six strains were isolated by using the dilution plating method on starch casein nitrate agar plate and potato starch-glycerol agar plate. On the basis of phenotypic characteristics and the 16S rRNA gene sequence analysis, they were belonged to the genus Streptomyces and were identified as S. tendae (5 isolates), S. malachitospinus (2 isolates), S. marokkonensis (2 isolates), S. parvulus (2 isolates), S. fragilis (1 isolate), S. diastaticus (1 isolate), S. drozdowiczii (1 isolate), S. olivochromogenes (1 isolate), S. aureus (2 isolates), S. iranensis (1 isolate), S. rapamycinicus (1 isolate), S. yatensis (1 isolate), S. samsunensis (4 isolates), S. spiralis (2 isolates). On the antimicrobial activity screening, 15 isolates exhibited activity against Bacillus subtilis ATCC 6633, 13 isolates did against Kocuria rhizophila ATCC 9341, 6 isolates against Mucor racemosus IFO 4581 and Candida albicans KF1, one isolate against Escherichia coli NIHJ KB213 and 5 isolates against Xanthomonas campestris pv. oryzae KB88.