Topic
Kinesin complex
About: Kinesin complex is a research topic. Over the lifetime, 18 publications have been published within this topic receiving 1617 citations.
Papers
TL;DR: Most major categories of proteins in the human proteome are represented in plasma, with the distribution at successively deeper layers shifting from mostly extracellular to a distribution more like the whole (primarily cellular) proteome.
1,153 citations
TL;DR: It is found that Dam1 binds to the plus end of spindle microtubules and kinetochores as cells enter mitosis and this is dependent on other components of the fission yeast DASH complex, including Ask1, Duo1, Spc34 and Dad1.
Abstract: We identified a truncated allele of dam1 as a multicopy suppressor of the sensitivity of cdc13-117 (cyclin B) and mal3-1 (EB-1) cells to thiabendazole, a microtubule poison. We find that Dam1 binds to the plus end of spindle microtubules and kinetochores as cells enter mitosis and this is dependent on other components of the fission yeast DASH complex, including Ask1, Duo1, Spc34 and Dad1. By contrast, Dad1 remains bound to kinetochores throughout the cell cycle and its association is dependent on the Mis6 and Mal2, but not Mis12, Nuf2 or Cnp1, kinetochore proteins. In cells lacking Dam1, or other components of the DASH complex, anaphase is delayed due to activation of the spindle assembly checkpoint and lagging sister chromatids are frequently observed and occasionally sister chromatid pairs segregate to the same spindle pole. We find that the mitotic centromere-associated Klp5/Klp6 kinesin complex is essential in cells lacking components of the DASH complex. Cells lacking both Dam1 and Klp5 undergo a first cell cycle arrest in mitosis due to a failure to establish bipolar chromosome attachment.
142 citations
TL;DR: It is reported here that the hyperphosphorylation of components of the kinesin complex by treatment with okadaic acid increases kinesIn motor activity at least 2-fold, and it is concluded thatKinesin motor activity is directly modulated by the phosphorylation state of kines in-associated proteins.
89 citations
TL;DR: The results suggest that the FEZ1/kinesin complex functions for the transport of mitochondria along microtubules toward the extending neurites in differentiating PC12 cells.
84 citations
TL;DR: An atomic resolution model of the motor domain dimer is built which can be successfully 'docked' into the three-dimensional framework of the maps from electron cryomicroscopy and X-ray crystallographic data.
77 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 1 |
| 2019 | 1 |
| 2018 | 2 |
| 2015 | 2 |
| 2014 | 2 |
| 2013 | 1 |