Topic
J-segment
About: J-segment is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 32 citations. The topic is also known as: sport utility cars.
Papers
TL;DR: The results show that a spontaneously arising and an antigen‐induced autoantibody use an identical VDJ gene rearrangement and that after hyperimmunization somatic mutation did not occur.
Abstract: We report on the molecular characterization of the heavy (H) chain variable (V) region of two murine autoantibodies reacting with a conventional self antigen, thyro-globulin (Tg), originated from unimmunized/unstimulated neonatal mice (clone B10H2) and from an adult hyperimmunized mouse (clone 62), respectively. Serologically, both hybridoma antibodies express the same idiotype (Id). By cloning and sequencing we demonstrated that their VH regions are encoded by identical VDJ gene elements including the VD and DJ joints. This VH belongs to the VH 7183 gene family, the most 3′-end proximal family in the murine genome. The D segment is unique and only 50% similar to any murine D segment. The J segment utilized is germ-line JH4. The cloned DNA rearrangement was transfected into the J558L myeloma cells and the secreted antibody was found to express the reference Id on the H chain, hence proving that the productive VDJ rearrangement had been identified. These results show that a spontaneously arising and an antigen-induced autoantibody use an identical VDJ gene rearrangement and that after hyperimmunization somatic mutation did not occur. The significance of this finding with respect to ontogeny of the B cell repertoire is discussed.
34 citations
1 Jan 1986
TL;DR: The inversion chromosome abnormality in this cell line results in the formation of a chimaeric gene comprising both immunoglobul in and T cell receptor DNA segments, and the possible relationship of this fused gene to tumour aetiology is discussed.
Abstract: Studies on the organisation and rearrangement of the human T-cell receptor α chain locus shows that the single constant region gene is associated with multiple joining segments dispersed over a region of at least 20 kb. The locus maps to the long arm of human chromosome 14 at band 14qll. Genomic clones isolated from a cell line derived from a T cell lymphoma with the marker chromosome inv 14 (14qll;q32) showed that one allele has a Jα segment non-productivity rearranged with an α variable region segment but the other allele has a productive rearrangement between an α gene J segment and a variable region from the immunoglobulin locus. This fusion gene occurs at the inversion 14 chromosomal breakpoint. Therefore the inversion chromosome abnormality in this cell line results in the formation of a chimaeric gene comprising both immunoglobul in and T cell receptor DNA segments. The possible relationship of this fused gene to tumour aetiology is discussed.
TL;DR: This article found evidence for a substantial proportion of TCRβ rearrangements lacking a D-segment in the naive repertoire, and such sequences are preferentially generated during fetal development and persist within the elderly.
Abstract: T cells play an important role in adaptive immunity. An enormous clonal diversity of T cells with a different specificity, encoded by the T cell receptor (TCR), protect the body against infection. Most TCRβ chains are generated from a V, D, and J segment during recombination in the thymus. Although complete absence of the D segment is not easily detectable from sequencing data, we find convincing evidence for a substantial proportion of TCRβ rearrangements lacking a D segment. Additionally, sequences without a D segment are more likely to be abundant within individuals and/or shared between individuals. Our analysis indicates that such sequences are preferentially generated during fetal development and persist within the elderly. Summarizing, TCRβ rearrangements without a D segment are not uncommon, and tend to allow for TCRβ chains with a high abundance in the naive repertoire.
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 1989 | 1 |
| 1986 | 1 |