Topic
Isoptericola jiangsuensis
About: Isoptericola jiangsuensis is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 26 citations.
Papers
TL;DR: The results showed that strain CLG(T) represents a novel species of the genus Isoptericola, for which the name Isptericola jiangsuensis sp.
Abstract: A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium,
strain CLGT, was isolated from beach sand from Lianyungang, Jiangsu
Province, PR China. The organism grew optimally at 30 °C and pH 7.0.
Strain CLGT contained l-Lys–d-Asp as
the peptidoglycan type and galactose, rhamnose and xylose as the whole-cell
sugars. It contained anteiso-C15 : 0 and iso-C15 : 0 as the major fatty acids. The major polar lipids
were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidylinositol,
three phosphoglycolipids and one unidentified lipid. The DNA G+C content
was 70.3 mol%. The 16S rRNA gene sequence similarity of strain
CLGT with Isoptericola dokdonensis DS-3T was
99.4 % but the DNA–DNA relatedness between strain CLGT and I. dokdonensis DS-3T was 58.7 %.
There were also many phenotypic properties that distinguished strain CLGT from recognized species of the genus Isoptericola. The results
showed that strain CLGT represents a novel species of the genus Isoptericola, for which the name Isoptericola jiangsuensis sp.
nov. is proposed. The type strain is CLGT (=DSM 21863T=CCTCC AB208287T).
23 citations
TL;DR: Is-chiA and Is-chiB genes were cloned into expression vector pET28a (+) and expressed in Escherichia coli BL21 (DE3) with isopropyl-β-d-thiogalactopyranoside induction and showed chitobiosidase and endochitinase activity, respectively.
Abstract: A 274-bp conserved fragment of chiA (chiA-CF) was amplified from the genomic DNA of Isoptericola jiangsuensis CLG (DSM 21863, CCTCC AB208287) using the specific PCR primers. Based on chiA-CF sequences, a 5233-bp DNA fragment was obtained by self-formed adaptor PCR. DNA sequencing analysis revealed there were two contiguous open reading frames coding for the precursors of Is-chiA [871 amino acids (aa)] and Is-chiB (561 aa) in the 5233-bp DNA fragment. The Is-chiA and Is-chiB exhibited 58% and 62% identity with ArChiA and ArChiB chitinase from Arthrobacter sp. TAD20, respectively. The Is-chiA and Is-chiB genes were cloned into expression vector pET28a (+) and expressed in Escherichia coli BL21 (DE3) with isopropyl-β-d-thiogalactopyranoside induction. Is-chiA and Is-chiB were 92 kDa and 60 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and showed chitobiosidase and endochitinase activity, respectively. Is-chiA and Is-chiB were purified by Ni-nitrilotriacetic acid affinity chromatography and the characteristics of both Is-chiA and Is-chiB were studied.
8 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2011 | 1 |
| 2010 | 1 |