TL;DR: A collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases, and the best genera for lipase production were Cladosporium and Penicillium.
Abstract: Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed the production of extracellular amylases, cellulases, proteases and lipases of 33 of those fungal isolates. The enzymatic activities were evaluated by the formation of a clear halo or a white precipitate around the colonies in defined substrate media. The found fungi belong to the genera Talaromyces, Stenocarpella, Penicillium, Phlebiopsis, Cladosporium, Hyphopichia, Epicoccum, Trichoderma, Aspergillus, Irpex, Fusarium, Microdochium, Mucor and Sarocladium. In the genus Fusarium, the species Fusarium verticillioides was predominant and this genus presented the highest diversity, followed by the genera Aspergillus. The best genera for lipase production were Cladosporium and Penicillium; while Cladosporium, Aspergillus and Penicillium were best for cellulase activity; Hyphopichia, Aspergillus and Irpex for amylase activity; and Cladosporium and Sarocladium for proteases activity. In conclusion, a collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases.
TL;DR: In the present paper Irpex is maintained as a monotypic genus, while Steccherinum is accepted with 17 species, of which S. willisii is described as new species and S. hydneum is proposed as a new combination.
Abstract: In the present paper Irpex is maintained as a monotypic genus, while Steccherinum is accepted with 17 species. Of these S. ethiopicum, S. galeritum, S. gilvum, S. lanestre, and S. willisii are described as new species, S. hydneum is proposed as a new combination. A considerable proportion of the paper is devoted to all the species (and a few subspecific taxa) that have been wrongly described in or transferred to Irpex and Steccherinum. Some of the specific epithets are transferred to other genera, such as Beenakia (p. 555), Climacodon (p. 546), Dentipellis (p. 551, 558) and Hyphodontia (p. 567).
TL;DR: Results indicate that the xylanase activity of F-1 is intrinsic to the cellulase itself, as well as those calculated from the Michaelis-Menten treatment of two substances competing for the same active site of the enzyme.
Abstract: An endo-cellulase [EC 3.2.1.4.] of carboxymethyl-cellulase type (F-1) which was fractionated from culture filtrate of Irpex lacetus and purified to electrophoretic and ultracentrifugal homogeneity, was found to show xylanase [EC 3.2.1.8.] activity. The activity was not removed from any of the intermediate fractions during the purification of the initial F-I peak, and the radio of xylanase to cellulase activity remained almost unchanged through the purification processes. The xylanase activity of F-I showed not only the same optiomal pH, heat stability, and pH stability as its cellulase activity, but also the same mobility as the cellulase activity upon cellulose acetate film and starch zone electrophoreses. The overall rates of hydrolysis of mixtures of variouis concentrations of CM-cellulose and xylan by F-1 coincided well with those calculated from the Michaelis-Menten treatment of two substances competing for the same active site of the enzyme. These results indicate that the xylanase activity of F-1 is intrinsic to the cellulase itself.
TL;DR: Type specimens of fifteen, resupinate, crustose basidiomycetes with aculei described by various authors were examined and X. fimbriiformis is accepted as a distinct species.
Abstract: Type specimens of fifteen, resupinate, crustose basidiomycetes with aculei described by various authors were examined. Nine taxa are later synonyms: Hydnum albiceps Berk. & Rav. (= Phlebia fascicularis), Hydnum chrysodon Berk. & M.A. Curtis (= Hydnophlebia chrysorhiza), Hydnum hypochrum Berk. & Broome (= Dentipellis leptodon), Hydnum micheneri Berk. (= Steccherinum ciliolatum), Hydnum pallidum Cooke & Ellis (= Xylodon fimbriiformis), Hydnum schweinitzii Berk. & M.A. Curtis (= H. chrysorhiza), Irpex furfuraceovelutinus Rick (= Kneiffiella stereicola), Irpex galzinii Bres. (= Amyloporia sinuosa), and Merulius elliottii Massee (= H. chrysorhiza). Irpex fimbriiformis Berk. & M.A. Curtis (≡ X. fimbriiformis) is accepted as a distinct species. Five new combinations are proposed: Cristinia decolorans for Irpex decolorans Cooke, Dentipellis ohiensis for Hydnum ohiense Berk., an earlier name for Dentipellis dissita (Berk. & Cooke) Maas Geest., Odonticium depauperatum for Irpex depauperatus Berk. & Broome, Peniophorella tessulata for Kneiffia tessulata Berk. & M.A. Curtis, an earlier name for Peniophorella rudis (Bres.) K.H. Larss., and Schizopora archeri for Irpex archeri Berk.