TL;DR: The combined morphological and chemical data indicated that hybridization was occurring and that, in contrast to previous reports, introgression was taking place in each of the parental species.
Abstract: Iris tenax andI. chrysophylla were collected along a linear transect extending through narrow areas of sympatry from two sites in the Willamette River drainage of western Oregon. Statistical analysis of correlations between morphological characteristics was used to construct a hybrid index for each species. Morphological evaluation and 2-dimensional paper chromatography of the major flavonoid constituents were prepared on a plant by plant basis along the transects. The combined morphological and chemical data indicated that hybridization was occurring and that, in contrast to previous reports, introgression was taking place in each of the parental species.
TL;DR: Results showed that <5% of I. gormanii ovules develop into seeds with open pollination, supporting earlier reports of low seed set and suggesting that pollen may be limiting in nature.
Abstract: Ovule development and ovule and fruit success were investigated in Iris tenax var. tenax and I. tenax var. gormanii. Ovule development, including megasporogenesis and initial stages of megagametogenesis, occurred while flowers were still in bud. Final maturation of the seven-celled embryo sac occurred during the male phase of flowering. An earlier report that synergids persist after fertilization, and that nucellar nuclei migrate into the developing megagametophyte in I. tenax var. tenax, was not supported in the present study. Reproductive studies used two pollination treatments: outcrossing and selfing. Treatment results were compared with results from open pollination. Both varieties of I. tenax are self-compatible. Results showed that <5% of I. tenax var. gormanii ovules develop into seeds with open pollination, supporting earlier reports of low seed set. Hand pollinations improved reproductive success, suggesting that pollen may be limiting in nature.
TL;DR: In this article, the authors developed germination protocols for greenhouse propagation of I. tenax (Oregon iris) in order to support plant production for use in habitat restoration in western Oregon and Washington.
Abstract: Grassland prairies of western Oregon and Washington are among the most endangered ecosystems in the United States. Active management and restoration are needed to promote biodiversity in the region. To support plant production for use in habitat restoration, we developed germination protocols for greenhouse propagation of Iris tenax (Oregon iris). Dormancy was most effectively overcome (63% germination) by four weeks of warm stratification at 20/30°C followed by 6–12 weeks of cold stratification at 5°C suggesting that I. tenax may have morphophysiological dormancy. This result was consistent across multiple source populations.
TL;DR: A phytochemical profile of a botanical product (TM) from Iris tenax has been produced focused on flavonoids and iridals, leading to a useful fingerprint of the tested extract based on HPLC and NMR data.
Abstract: A phytochemical profile of a botanical product (TM) from Iris tenax has been produced focused on flavonoids and iridals, leading to a useful fingerprint of the tested extract based on HPLC and NMR data.