Topic
Inosculation
About: Inosculation is a research topic. Over the lifetime, 33 publications have been published within this topic receiving 2223 citations. The topic is also known as: Anastomosis (botany) & gemel.
Papers
TL;DR: This work has developed the first endothelialized human tissue‐engineered skin in which a network of capillary‐like tubes is formed, and the ESE turns out to be a promising in vitro angiogenesis model.
Abstract: For patients with extensive burns, wound coverage with an autologous in vitro reconstructed skin made of both dermis and epidermis should be the best alternative to split-thickness graft. Unfortunately, various obstacles have delayed the widespread use of composite skin substitutes. Insufficient vascularization has been proposed as the most likely reason for their unreliable survival. Our purpose was to develop a vascular-like network inside tissue-engineered skin in order to improve graft vascularization. To reach this aim, we fabricated a collagen biopolymer in which three human cell types keratinocytes, dermal fibroblasts, and umbilical vein endothelial cells were cocultured. We demonstrated that the endothelialized skin equivalent (ESE) promoted spontaneous formation of capillary-like structures in a highly differentiated extracellular matrix. Immunohistochemical analysis and transmission electron microscopy of the ESE showed characteristics associated with the microvasculature in vivo (von Willebrand factor, Weibel-Palade bodies, basement membrane material, and intercellular junctions). We have developed the first endothelialized human tissue-engineered skin in which a network of capillary-like tubes is formed. The transplantation of this ESE on human should accelerate graft revascularization by inosculation of its preexisting capillary-like network with the patient's own blood vessels, as it is observed with autografts. In addition, the ESE turns out to be a promising in vitro angiogenesis model.
480 citations
Patent•
29 Jan 1986
TL;DR: An incision opening expansion holder for use surgical operation, especially for inosculation comprising an outer tube being capable of inserting into the lumen of a vein graft, an inner tube sliding into the outer tube and a grip being fixed to one end of the inner tube.
Abstract: An incision opening expansion holder for use surgical operation, especially for inosculation comprising an outer tube being capable of inserting into the lumen of a vein graft, an inner tube slidably inserting into the outer tube and a grip being fixed to one end of the inner tube, the inner tube has a plurality of short wires, the bases of which are fixed to the other end of the inner tube and each wire is to be energized to expand so as to hold the incision opening of the vein graft open when the holder is inserted into the lumen and also when the wires of the inner tube are projected out of the outer tube by handling the grip, so that inosculation operation can be undertaken under good visual field and surgeons can observe easily the piercing point of sewing of the vein graft.
401 citations
TL;DR: It is concluded that the early vascularization observed in the ERS was most probably the result of inosculation of the CLS network with the host's capillaries, rather than neovascularization, which is a slower process.
397 citations
TL;DR: During the last years, considerable progress has been made in the development of promising vascularization strategies in tissue engineering, and particularly the inosculation of preformed microvascular networks has the great potential to markedly improve the survival of tissue constructs after implantation.
Abstract: Background/Purpose: The key challenge in tissue engineering is the establishment of an efficient vascularization for tissue constructs guaranteeing long-term survival and function.
207 citations
TL;DR: With the use of sophisticated in vitro and in vivo models, more detailed analysis of regulatory mechanisms of inosculation will help to develop novel strategies, aiming at further accelerating the establishment of a life-sustaining blood supply to implanted tissue constructs.
Abstract: Recent progress in engineering microvascular networks in vitro and in vivo offers exciting opportunities to create tissue constructs with preformed blood vessels, which are rapidly blood perfused by developing interconnections to the preexisting blood vessels of the host tissue after implantation. This process, termed as inosculation, is well known from the revascularization of various tissue grafts, such as transplanted skin, nerves, or bone. It is characterized by the close interaction of the implant's preformed microvascular network and the host microvasculature. The sprouting angiogenic activity of both counterparts determines whether inosculation takes place internally within the implant or externally within the surrounding host tissue. Successful inosculation involves vascular remodeling as well as infiltration of inflammatory cells and stem cells. With the use of sophisticated in vitro and in vivo models, more detailed analysis of regulatory mechanisms of inosculation will help to develop novel strategies, aiming at further accelerating the establishment of a life-sustaining blood supply to implanted tissue constructs.
150 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2018 | 2 |
| 2016 | 2 |
| 2015 | 1 |
| 2014 | 2 |
| 2013 | 1 |