Topic
Infranatant
About: Infranatant is a research topic. Over the lifetime, 18 publications have been published within this topic receiving 593 citations.
Papers
TL;DR: The effects of LDL were not cell specific because normal as well as LDL receptor negative human skin fibroblasts were injured by LDL, and an injurious effect of a fixed concentration of LDL could be completely prevented by increasing the concentration of infranatant proteins.
Abstract: Low density lipoproteins (LDL) have been shown to injure culture endothelial cells derived from the human umbilical cord. During a 48 h incubation period LDL significantly increased 51Cr release from prelabelled cells and induced marked cellular injury if the ratio between the LDL cholesterol and the infranatant proteins was kept above 0.1-0.12 mmol/g protein. Actually, an injurious effect of a fixed concentration of LDL could be completely prevented by increasing the concentration of infranatant proteins. High density lipoproteins within physiological concentration ranges had no effect when tested in the presence of infranatant proteins. The effects of LDL were not cell specific because normal as well as LDL receptor negative human skin fibroblasts were injured by LDL.
246 citations
TL;DR: Cryo-transmission electron microscopy shows that the infranatants contain vesicles (mostly unilamellar) at the side of residual small emulsion particles, consistent with the distribution of phospholipids between outer and inner lamellae, as determined by 31P-NMR.
74 citations
TL;DR: The effect of hyperlipidemic rat serum and its fractions on the synthetic functions of embryonic fibroblasts was studied and the stimulating principle was nondialyzable.
39 citations
TL;DR: An ultracentrifugation method for isolating the different lipoprotein classes relatively quickly, including anomalous lipoproteins such as the IDL, is described.
Abstract: We describe an ultracentrifugation method for isolating the different lipoprotein classes relatively quickly. In this method the very-low-density lipoproteins are first separated by non-density-adjusted ultracentrifugation. The resulting infranatant material is then stained with Coomassie Brilliant Blue R-250 and ultracentrifuged in a density gradient. The intermediate-density lipoproteins (IDL), low-density lipoproteins, and high-density lipoproteins fractions are separated by aspiration from the top of the tube. This method can be used to separate, analyze, and quantify lipoproteins, including anomalous lipoproteins such as the IDL. The CVs for the present method never exceeded 15%.
37 citations
TL;DR: Cultured human endothelial cells preincubated with the infranatant of human serum increased their content of cholesterol when subsequently exposed to low density lipoproteins as compared to control cultures further incubated in the presence of infran atant only.
21 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2018 | 1 |
| 2016 | 1 |
| 2015 | 1 |
| 2009 | 1 |
| 2005 | 1 |
| 1992 | 1 |