Topic
Hydroxynitrilase
About: Hydroxynitrilase is a research topic. Over the lifetime, 1 publications have been published within this topic. The topic is also known as: hydroxynitrile lyase.
Papers
TL;DR: In this article, the synthesis of chiral silicon-containing (R)-ketone-cyanohydrin by enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin was efficiently carried out using defatted Prunus domestic seed meal as (R]-hydroxynitrilase lyase for the first time.
Abstract: The synthesis of chiral silicon-containing (R)-ketone-cyanohydrin by enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin was efficiently carried out using defatted Prunus domestic seed meal as (R)-hydroxynitrilase lyase for the first time. Under the optimal conditions including a reaction temperature of 25-35°C and a buffer pH of 5.0-5.4, both acetyltrimethylsilane conversion and enantiomeric excess of the product were above 99%. In contrast, this enzyme did not accepted its carbon counterpart 3,3-dimethyl-2-butanone as substrate. These results demonstrated that the silicon atom in substrate served as a more effective atom than the carbon atom to enhance the activity of the enzyme.
Performance Metrics
| Year | Papers |
|---|---|
| 2011 | 1 |