TL;DR: Flower and inflorescence anatomy and morphology of Exostyles, Harleyodendron, Holocalyx, Lecointea, and Zollernia (Leguminosae) were studied and anatomical characters are discussed according to their possible phylogenetic implications.
Abstract: Flower and inflorescence anatomy and morphology of Exostyles, Harleyodendron, Holocalyx, Lecointea, and Zollernia (Leguminosae, Lecointea clade) were studied. Features common to all genera but otherwise rare within the Leguminosae include: (1) the presence of phenolic compounds in the epidermal cells of the anthers and subepidermal cells of the bracteoles, sepals, petals, and ovaries (absent in Holocalyx balansae); (2) simple trichomes on the adaxial base of the bracteoles and on the surface of the calyx and ovaries; and (3) tapetum persisting until the androspores are formed. Other notable anatomical features are: (1) colleters on the adaxial bases of the bracts and bracteoles of Holocalyx balansae and Zollernia ilicifolia; (2) trichomes on the anthers of Harleyodendron unifoliolatum, Holocalyx balansae, Lecointea hatschbachii, Zollernia ilicifolia and Z. magnifica; (3) osmophores on the petals of Exostyles godoyensis; (4) asynchronous pollen development in the anthers of Holocalyx balansae and Zollernia magnifica; and (5) vascular bundles surrounded by lignified fibers in Harleyodendron unifoliolatum. These anatomical characters are discussed according to their possible phylogenetic implications.
TL;DR: The morpho-anatomy of the leaf and stem of this native medicinal species of Holocalyx balansae Micheli is studied in order to increase knowledge of the Brazilian flora and to contribute to pharmacognostic quality control.
Abstract: Holocalyx balansae Micheli belongs to the Fabaceae family and is called pau-alecrim in Portuguese. In folk medicine, it is indicated to treat heart, hepatic and digestive upsets, as well as being considered anti-septic and diaphoretic. This work aimed to study the morpho-anatomy of the leaf and stem of this native medicinal species, in order to increase knowledge of the Brazilian flora and to contribute to pharmacognostic quality control. Samples of mature leaves and young stems were fixed and either sectioned free-hand or embedded in glycol-methacrylate and sectioned by microtome, then stained. Microchemical tests and scanning electron microscopy were also performed. The leaves are alternate, compound and paripinnate, and the leaflets have a lanceolate shape. Anomocytic and anisocytic stomata are found exclusively on the abaxial surface. The mesophyll is dorsiventral and the midrib, in cross-section, is flat on both sides, showing one collateral vascular bundle, encircled by a sclerenchymatic and crystalliferous sheath. The rachis and petiole present a collateral vascular bundle with a centric arrangement. The stem presents phellogen localized superficially. In the vascular cylinder, there is a sclerenchymatic sheath and continuous phloem and xylem cylinders, both traversed by narrow rays. Prismatic crystals of calcium oxalate are present in the leaf and stem.
TL;DR: It is concluded that Holocalyx is monotypic, containing the single species H. balansae.
Abstract: The tree genus Holocalyx (Leguminosae, Papilionoideae) is distributed in southern South America and inhabits moist forests or gallery forests of the Cerrado. It is poorly studied taxonomically in spite of its economic importance as material for tool handles, rustic furniture, and fuel wood. Holocalyx is characterized by flowers with a nearly truncate calyx, an actinomorphic corolla of five petals, an androecium of ten stamens, and a stipitate gynoecium positioned centrally within the flower. There are two binomials in the genus: Holocalyx balansae and H. glaziovii. The first one was introduced with the description of the genus, but type material was not specified for the species. The latter, despite being a nomen nudum, is relatively widely applied in herbarium identifications. In this paper, we evaluate nomenclature within Holocalyx and present a taxonomic revision of the genus. We conclude that Holocalyx is monotypic, containing the single species H. balansae.