Gyrinops

Abstract: Aromatics originating from the resin-infused infected wood of the Aquilaria and Gyrinops genera have distinct and valued fragrances. Resin formation occurs as a response to internal injury and/or infections in the stems of the agarwood tree. The incenses and perfumes that are produced from agarwood have been valued for centuries and used by many cultures for spiritual, opulent, and aphrodisiac purposes. Agarwood is highly revered in the seminal texts of Hinduism, Christianity, Buddhism, and Islam. As early as 1400 B.C.E., agarwood was described as a fragrant product in Sanskrit texts, and in 65 B.C.E., Dioscorides detailed several medical applications for agarwood. Knowledge of human-induced agarwood formation was recorded in China as early as 300 C.E., where it was known that cutting into a tree would lead to a color change in its internal tissues within a year of the injury as a consequence of resin development. There is historical evidence of international trade in agarwood between the producing (tropical southeastern Asia) and consuming (Middle East, India, China, and Japan) regions of the world. Agarwood has been consumed primarily as incense with the burning of wood chips directly or as an important ingredient for powdered incense blends. The aromatic qualities of agarwood are influenced by the species and variety of the two genera, geographic location, its branch, trunk and root origin, length of time since infection, and methods of harvesting and processing. The subtle but numerous variations in agarwood properties led to the development of systems for product classification and description in several consumer countries. This paper reviews the uses and trade of agarwood and its social, political, and economic significance in human history.

Abstract: RATIONALE: It is important for the enforcement of the CITES treaty to determine whether agarwood (a resinous wood produced in Aquilaria and Gyrinops species) seen in trade is from a plantation that was cultivated for sustainable production or was harvested from natural forests which is usually done illegally. METHODS: We analyzed wood directly using Direct Analysis in Real Time (DART ™ ) ionization coupled with Time-ofFlight Mass Spectrometry (TOFMS). Agarwood was obtained from five countries, and the collection contained over 150 samples. The spectra contained ions from agarwood-specific 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromones as well as many other ions. The data was analyzed using either kernel discriminant analysis or kernel principal component analysis. Probability estimates of origin (wild vs cultivated) were assigned to unknown agarwood samples. RESULTS: Analysis of the DART-TOFMS data shows that many of the chromones found in cultivated and wild agarwood samples are similar; however, there is a significant difference in particular chromones that can be used for differentiation. In certain instances, the analysis of these chromones also allows inferences to be made as to the country of origin. Mass Mountaineer ™ software provides an estimate of the accuracy of the discriminate model, and an unknown sample can be classified as cultivated or wild. Eleven of the thirteen validation samples (85%) were correctly assigned to either cultivated or wild harvested for their respective geographic provenance. The accuracy of each classification can be estimated by probabilities based on Z scores. CONCLUSIONS: The direct analysis of wood for the diagnostic chromones using DART-TOFMS followed by discriminant analysis is sufficiently robust to differentiate wild from cultivated agarwood and provides strong inference for the origin of the agarwood. Copyright © 2013 John Wiley & Sons, Ltd.

Abstract: Agarwood plays an important role in gaining foreign exchange and as a source of income for people living in, around, and inside the forest of Indonesia. Its production has declined rapidly due to the lack of proven technology for its induction. If no serious action is taken now, agarwood production will not be sustainable in the future. Agarwood formation is initiated by biotic and/or abiotic factors and is affected by host tree, microbe, and environment. Nonetheless, in cultivating agarwood on a large scale, a standard operating procedure is the principal factor that determines agarwood quantity and quality. Realizing the importance of a procedure for agarwood induction, we developed a technology using the biotic factor and its natural way in forming agarwood. We applied selected endophytic fungi directly on Aquilaria and Gyrinops trees. Because fungi are living organisms, naturally they help to spread the induction mechanism into other regions of the tree. Remarkably, the technology produced substantial amount of agarwood of high-grade quality. Although a better selection of fungal strains must be discovered and tested in field trials, this promising technology could be the answer to commercial agarwood production in Indonesia and could be adopted by other countries.

Abstract: The genera Aquilaria and Gyrinops (Thymelaeaceae, Malvales) are well known for the production of agarwood which is a highly wanted forest product of substantial economic value. The taxonomic status of Aquilaria and Gyrinops as separate genera is doubted as they are only distinguished by the number of stamens. We investigated their status by conducting phylogenetic analyses of DNA sequences from the plastid trnL-trnF spacer. Control of international trade of agarwood is currently hampered by the failure of traditional methods such as microscopy to identify samples to species level. We therefore evaluated the potential of molecular identification of agarwood by searching for species- and region-specific plastid DNA polymorphisms. DNA sequences were obtained from 31 Thymelaeaceae accessions encompassing 20 different species in six genera. Aquilaria and Gyrinops appear to be paraphyletic. Success in sequencing wood samples demonstrates that molecular markers provide new perspectives for agarwood identification.