TL;DR: The findings in this study indicate the possibility that the isolated strains produce novel acid beta-galactosidases that are able to hydrolyze lactose at low temperature.
TL;DR: Nucleotide sequence analyses of the hymenomycetous yeasts demonstrated that Hyalodendron lignicola should be considered as a member of the genus Trichosporon within the Trichsporonales and that TrichOSporon pullulans is associated closely with the Cystofilobasidiales, rather than the TrICHospor onales.
Abstract: Nucleotide sequence analyses of the hymenomycetous yeasts demonstrated that Hyalodendron lignicola should be considered as a member of the genus Trichosporon within the Trichosporonales and that Trichosporon pullulans is associated closely with the Cystofilobasidiales, rather than the Trichosporonales. Accordingly, the following proposals are made: Trichosporon lignicola comb. nov. and Guehomyces gen. nov., to accommodate Guehomyces pullulans comb. nov. in the Cystofilobasidiales.
TL;DR: The matrix-assisted laser desorption/ionization time-of-flight/time- of-flight mass spectroscopy identified a peptide ALEEYKK which is the conserved motif of the β-galactosidases from other yeasts, showing that the enzyme may have potential applications in food industry.
TL;DR: A psychrotolerant yeast Guehomyces pullulans 17-1 was isolated from sea sediment in Antarctica that could yield both extracellular and cell-bound β-galactosidase and a large amount of reducing sugar could be released from milk under catalysis of the yeast culture.
Abstract: A psychrotolerant yeast Guehomyces pullulans 17-1 was isolated from sea sediment in Antarctica. It was found that it could yield both extracellular and cell-bound β-galactosidase. After optimization of the medium and cultivation conditions, it was found that the yeast strain produced over 17.2 U mL⁻¹ of β-galactosidase activity within 120 h at the flask level while the yeast strain produced over 25.3 U mL⁻¹ of β-galactosidase activity within 144 h during the 2-L fermentation. This is the highest β-galactosidase activity produced by the wild type yeast strains reported so far. However, the optimal pH and temperature for the crude β-galactosidase were 4.0 and 50 °C, respectively. Lactose could be converted into glucose and galactose and a large amount of reducing sugar could be released from milk under catalysis of the yeast culture.
TL;DR: A total of 28 yeast strains obtained from the sea sediment of Antarctica have wide potential applications in biotechnology, for some of them can produce β-galactosidase and killer toxins.
Abstract: A total of 28 yeast strains were obtained from the sea sediment of Antarctica. According to the results of routine identification and molecular characterization, the strains belonged to species of Yarrowia lipolytica, Debaryomyces hansenii, Rhodotorula slooffiae, Rhodotorula mucilaginosa, Sporidiobolus salmonicolor, Aureobasidium pullulans, Mrakia frigida and Guehomyces pullulans, respectively. The Antarctica yeasts have wide potential applications in biotechnology, for some of them can produce β-galactosidase and killer toxins.