About: Growth hormone 2 is a research topic. Over the lifetime, 10 publications have been published within this topic receiving 296 citations. The topic is also known as: GH-V & GHL.
TL;DR: There was evidence that the transformed rainbow trout (Oncorhynchus mykiss) were unable to produce SsGH2 mRNA in their pituitary glands, and the presence of the transgene in various tissues was examined in trout to evaluate the reliability of one-tissue sampling.
TL;DR: Chromosome location: The location of the canine gene is unknown; the corresponding human gene maps to 17q21 (Law et a1. 1986).
Abstract: Chromosome location: The location of the canine gene is unknown; the corresponding human gene maps to 17q21 (Law et a1. 1986). or to 17ql1.1-q12 (Xu et a1. 1988). Acknowledgement: This work was supported by a grant from the American Kennel Club. References Hogg D. et a1. (1986J JBioI Chern 261,12420-7. Law M.L. et al. (1986J Cytogenet Cell Genet 42,202-7. Xu W. et al. (1988J Proc Nat! Acad Sci USA 85, 8563-7.
TL;DR: Results indicated that direct comparison of cellular responses between tissues from young, middle-aged, and old rats in primary cell culture is confounded by variations in time for recovery of optimal responses, the effects of the enzymes used for dispersal, and the methods used to express the data.
Abstract: Five criteria were developed to validate the primary cell culture model for comparison of GRF-induced release of growth hormone in pituitary tissue from aging animals. Pituitaries from young (5-mo), middle-aged (14-mo), and old (24-mo) male Fischer 344 rats were dispersed using either trypsin/trypsin inhibitor or dispase and compared with respect to the number of pituitary cells recovered, cell viability, 3H-leucine incorporation into total protein, time course for recovery of optimal response to GRF, and the dose-relationship for GRF-induced release of growth hormone 2, 4, and 6 days after dispersal. Results indicated that direct comparison of cellular responses between tissues from young, middle-aged, and old rats in primary cell culture is confounded by variations in time for recovery of optimal responses, the effects of the enzymes used for dispersal, and the methods used to express the data.