TL;DR: It is shown that the spatio-temporal distortion, pulse-front tilt, is naturally, easily, and sensitively measured by the recently demonstrated, extremely simple variation of single-shot second-harmonic generation frequency-resolved optical gating (SHG FROG): GRENOUILLE.
Abstract: We show that the spatio-temporal distortion, pulse-front tilt, is naturally, easily, and sensitively measured by the recently demonstrated, extremely simple variation of single-shot second-harmonic generation frequency-resolved optical gating (SHG FROG): GRENOUILLE. While GRENOUILLE traces are ordinarily centered on the zero of delay, a pulse with pulse-front tilt yields a trace whose center is shifted to a nonzero delay that is proportional to the pulse-front tilt. As a result, the trace-center shift reveals both the magnitude and sign of the pulse-front tilt—independent of the temporal pulse intensity and phase. The effects of pulse-front tilt can then easily be removed from the trace and the intensity and phase vs. time also retrieved, yielding a full description of the pulse in space and time.
TL;DR: The data suggest that the function as well as the structure of PACAP have been conserved during the evolution of amphibia to mammals.
Abstract: Pituitary adenylate cyclase-activating polypeptide (PACAP), a peptide of the glucagon-secretin-vasoactive intestinal polypeptide superfamily, was isolated in pure form from the brain of the European green frog, Rana ridibunda. The primary structure of the peptide indicates that evolutionary pressure to conserve the complete amino acid sequence has been very strong. Frog PACAP comprises 38 amino acid residues and contains only 1 substitution (isoleucine for valine at position 35) compared with human/ovine/rat PACAP. In the presence of the phosphodiesterase inhibitor isobutylmethylxanthine, synthetic ovine PACAP–(1–38) produced a dose-dependent increase in the concentration of cAMP in isolated frog anterior pituitary fragments (ED50 = 2.1 ± 0.6 × 10−7 M; mean ± SE; n = 6). Maximum stimulation (an ∼8-fold increase in concentration over basal values) was produced by 10−6 M peptide. The truncated form of PACAP [PACAP–(l–27)] also produced a dose-dependent increase in cAMP in frog anterior pituitary fragments, ...
TL;DR: High-conductance anion channels could be induced in previously quiet patches by 20–120 s depolarizations beyond +20 mV and then studied in the potential range from −80 to +60 mV for a long time, with complex gating modes and periods of rapid flickering.
Abstract: Membrane patches were excised from enzymatically dissocfated frog toe muscle. High-conductance anion channels could be induced in previously quiet patches by 20–120 s depolarizations beyond +20 mV and then studied in the potential range from −80 to +60 mV for a long time. From reversal potentials the estimated permeability ratiosPCl/PNa andPCl/Pglucuronate were near 3.5 and 4, respectively. There were probably 5 or more conductance levels (substates) for a single channel, the most common in symmetrical 110 mM NaCl being 260 and 70 pS at 10°C. Gating was complex, with rapid and slow events and several gating modes, including periods of rapid flickering. Channels closed reversibly at potentials more negative than −50 mV. The channel was blocked by application to the cytoplasmic face of tannic acid, gallic acid, and zinc but not of DIDS or 9-anthracene-carboxylic acid, and it was blocked by extracellular zinc.
TL;DR: This technique utilizes surface third-harmonic generation as the nonlinear-optical effect and, surprisingly, is the most sensitive third-order FROG geometry yet.
Abstract: We demonstrate what is to our knowledge the first frequency-resolved optical gating (FROG) technique to measure ultrashort pulses from an unamplified Ti:sapphire laser oscillator without direction-of-time ambiguity. This technique utilizes surface third-harmonic generation as the nonlinear-optical effect and, surprisingly, is the most sensitive third-order FROG geometry yet.