Green shield bug

Abstract: Green shield bug, Palomena prasina L. (Heteroptera: Pentatomidae) is a common pest species in Turkish hazelnut orchards. It feeds on hazelnut fruits and causes premature nut dropping in early season and later kernel damage during nut development. Controlling P. prasina is currently done by spraying insecticides that looks like the only option. Since hazelnut covers quite a large area in the country, possibility of using microbial control agents for controlling P. prasina is rather important. In this study, firstly hazelnut orchards were surveyed and 8 entomopathogenic fungi isolates were obtained from insects. Virulance of these isolates was evaluated against P. prasina nymphs in laboratory at 25°C and 90±5 RH. Isolates included 2 Simplicillium lamellicola, 4 Lecanicillium muscarium and one Beauveria bassiana and one Isaria fumosorosea. Isolates were bioassayed against nymphs. Dead individuals were counted daily following treatment for 12 days. Lethal time values (LT50 and LT90) for entomopathogenic fungi were calculated. LT50 and LT90 values for the experimented isolates ranged from 3.20 to 8.48 days and from 9.32 to 40.30 days, respectively. At the end of 12 days post treatment, mortality rates were above (83%) at all treatments. Highest mortality rates (98.00 and 95.00%) were observed in at the isolate of L. musacarium and B. bassiana, respectively. Entomopathogenic fungi could be promising agents for controlling P. prasina.

Abstract: The green shield bug, Palomena prasina (Hemiptera: Pentatomidae), is a pest of deciduous trees and shrubs throughout Turkey and is found in a large variety of habitats, including gardens. We investigated the facultative cultivable bacterial flora of 10 healthy and 5 dead green shield bugs, collected from the vicinity of Trabzon, Turkey, and tested them for insecticidal activity. Based on the conventional and molecular tests, 12 different bacteria were isolated and identified as Curtobacterium sp., Rhodococcus sp., Arthrobacter nicotinovorans, Arthrobacter oxydans, Agrococcus jejuensis, Pseudomonas poae, Raoultella terrigena, Serratia sp., Lysinibacillus sphaericus, Stenotrophomonas rhizophila, Bacillus thuringiensis and Microbacterium oxydans. Mortalities due to the application of 0.5 mL of L. sphaericus, B. thuringiensis and R. terrigena at a density of OD600 1.89 were 60%, 70% and 60%, respectively, on adult P. prasina. This indicates that facultative cultivable bacterial flora isolated from P. prasina ...

Abstract: Green shield bug (GSB) Palomena prasina (L.) causes significant yield and quality losses in hazelnut production of Turkey. Alive and dead adults of GSB were collected from the main hazelnut cultiva...

Abstract: (Received 9 October 2013; returned 4 November 2013; accepted 23 April 2014) The green shield bug, Palomena prasina (Hemiptera: Pentatomidae), is a pest of deciduous trees and shrubs throughout Turkey and is found in a large variety of habitats, including gardens. We investigated the facultative cultivable bacterial flora of 10 healthy and 5 dead green shield bugs, collected from the vicinity of Trabzon, Turkey, and tested them for insecticidal activity. Based on the conventional and molecular tests, 12 different bacteria were isolated and identified as Curtobacterium sp., Rhodococcus sp., Arthrobacter nicotinovorans, Arthrobacter oxydans, Agrococcus jejuensis, Pseudomonas poae, Raoultella terrigena, Serratia sp., Lysinibacillus sphaericus, Stenotrophomonas rhizophila, Bacillus thuringiensis and Microbacterium oxydans. Mortalities due to the application of 0.5 mL of L. sphaericus, B. thuringiensis and R. terrigena at a density of OD600 1.89 were 60%, 70% and 60%, respectively, on adult P. prasina. This indicates that facultative cultivable bacterial flora isolated from P. prasina have potential for microbial control of this pest.