TL;DR: This paper conducted weekly surveys of over-summering shorebirds at mudflats, mangroves, and sand beaches at the Kadalundi-Vallikkunnu Community Reserve on the southwest coast of India, 2005-2018.
Abstract: The Indian subcontinent is the primary wintering ground and stopover site for migratory shorebirds to refuel along the Central Asian and South Asian Flyways. Despite the conservation importance of this region for migratory shorebirds, we lack information on the distribution and abundance of over-summering shorebirds—migrants that remain on their wintering grounds during the breeding season—to evaluate the impacts of anthropogenic change on this poorly understood life history strategy. We conducted weekly surveys of over-summering shorebirds at mudflats, mangroves, and sand beaches at the Kadalundi-Vallikkunnu Community Reserve on the southwest coast of India, 2005–2018. We examined long-term patterns of over-summering shorebird abundance, species composition, and habitat use and estimated proportional changes in over-summering abundance of each shorebird species documented at this globally important site. Over the 14 yr study, we documented 7 over-summering species, including Lesser Sand Plover (Charadrius mongolus), Whimbrel (Numenius phaeopus), Greater Sand Plover (Charadrius leschenaultia), Kentish Plover (Charadrius alexandrinus), Common Sandpiper (Actitis hypoleucos), Ruddy Turnstone (Arenaria interpres), and Pacific Golden-Plover (Pluvialis fulva). Mean abundance and species richness were higher at mudflats than at mangroves and sand beaches, with fewer individuals and species observed over-summering after 2010. Mudflats also had the highest species diversity and evenness compared to mangroves and sand beaches. Management plans that aim to restore vulnerable mudflats and mangroves and to reduce anthropogenic threats such as sand mining and waste dumping are needed to prevent the loss of important over-summering, foraging habitat for migratory shorebirds in southern India and along the Central Asian and South Asian Flyways.
TL;DR: The Lesser Sand Plover Charadrius mongolus and greater sand plover C. leschenaultii both breed across central Asia and spend the non-breeding season in Africa, the Middle East, southern Asia and Australasia as discussed by the authors.
Abstract: The Lesser Sand Plover Charadrius mongolus and Greater Sand Plover C. leschenaultii both breed across central Asia and spend the non-breeding season in Africa, the Middle East, southern Asia and Australasia. Despite often being common and occurring in large numbers surprisingly little is known about their breeding and non-breeding distribution or migration routes, particularly for those migrating west to the Middle East and Africa. This paper presents new ringing recovery information relating to both species and suggests some possible options for their migration route between Kenya and their breeding grounds.
TL;DR: In this article, a molecular approach was used to determine the gender and genetic diversity of Charadrius spp. using intron length polymorphisms in chromo-helicase-DNA-binding (CHD) genes on both Z and W chromosomes.
Abstract: Charadrius spp. are migratory shorebirds in Thailand during the non-breeding season inwhich adult plumages are not clearly sexually dimorphic. In this work, a molecular approach was used to determine the gender and genetic diversity. Amplification of intron length polymorphisms in chromo-helicase-DNA-binding ( CHD ) genes on both Z and W chromosomes of three Charadrius spp. included 87 lesser sand plover ( Charadrius mongolus ), 27 greater sand plover ( C. leschenaultii ) and 3 kentish plover ( C. alexandrinus ). The resulting PCR products from 2550F/2718R primer showed fragments on a conventional agarose gel electrophoresis with size differences ranging from 200 bp between the two respective ZW allele. Males were identified by the presence of a single band about 650 bp ( CHD-Z ). Heterogametic females were identified by the presence of a second additional fragment length of approximately 450 bp ( CHD-W ). The CHD-Z fragments were purified and analysed by sequencing. The multiple sequence comparison was accomplished using ClustalX and PHYLIP software. These data demonstrate that the CHD-Z sequence related with morphology and can be used for genetic diversity study. The results of molecular sex determination and genetic diversity analyses will also have implications for shorebird conservation.
TL;DR: Genomic DNA was collected from blood samples of three Charadrius spp.
Abstract: Charadrius spp. are small shorebirds which wintering in Thailand during non-breeding period. outside the breeding season the adult is nearly sexually monomorphic that sex is difficult to identify. Therefore, sex identification using molecular technique is recommended. In this study, using FTA ® card, genomic DNA was collected from blood samples of three Charadrius spp., including lesser sand plover (Charadrius mongolus), greater sand plover (C. leschenaultii) and kentish plover (C. alexandrinus). The 2550F/2718R primer can be used to amplify the intronic region of the chromo-helicase-DNA binding (CHD) gene and the resulting PCR products were analyzed by agarose gel electrophoresis. The females produced two bands, which are 450 bp (CHD-W) and around 650 bp (CHD-Z), whereas samples from the males produced only a single band at around 650 bp (CHD-Z). Additionally, the nucleotides sequence of CHD-Z of C. alexandrines is different from C. mongolus and C. leschenaultii is 13 bp.