Topic
Glutamine binding
About: Glutamine binding is a research topic. Over the lifetime, 150 publications have been published within this topic receiving 5355 citations.
Papers published on a yearly basis
Papers
TL;DR: It is reported that the fluorescent amino acid, 3-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair.
Abstract: Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.
224 citations
TL;DR: The crystal structure of the GlnBP-Gln was determined and refined to 1.94 A resolution in this article, and the most significant changes were 41.1° in the φ angle of Gly89 and 34.3° in Glu181 from the first and second hinge of the protein, respectively.
194 citations
TL;DR: It is shown that, at least in this case, it is possible to predict one conformational state of a periplasmic binding protein from another conformationalstate of the protein.
174 citations
TL;DR: A role for the glutamine binding protein in active transport is suggested by the fact that the initial rate of glutamine uptake is reduced 90% by osmotic shock while certain other transport systems are maintained, and this is associated with release of the glutamines binding protein.
159 citations
TL;DR: In this article, high-resolution structures of the ligand binding core of GluR0, a glutamate receptor ion channel from Synechocystis PCC 6803, have been solved by X-ray diffraction.
152 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 6 |
| 2019 | 6 |
| 2017 | 4 |
| 2016 | 2 |
| 2015 | 5 |
| 2014 | 3 |