Topic
Genetic analysis
About: Genetic analysis is a research topic. Over the lifetime, 1890 publications have been published within this topic receiving 56171 citations.
Papers published on a yearly basis
1 / 2
Papers
TL;DR: A method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants is developed, which should produce a global map of gene function.
Abstract: In Saccharomyces cerevisiae, more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization (BNI1,ARP2, ARC40, BIM1), DNA synthesis and repair (SGS1, RAD27), or uncharacterized functions (BBC1, NBP2) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.
2,328 citations
Journal Article•
1,349 citations
Book•
1 Jan 2001TL;DR: In this article, the authors present a brief history of animals and their development, including the genetic toolkit for development. But they do not discuss the evolution of the toolkit itself.
Abstract: Preface.1. A Brief History of Animals.2. The Genetic Toolkit for Development.3. Building Animals.4. Evolution of the Toolkit.5. Diversification of Body Plans and Body Parts.6. Evolution of Morphological Novelties.7. Morphological Variation and Species Divergence.8. From DNA to Diversity: The Primacy of Regulatory Evolution.Glossary.Index
957 citations
TL;DR: It is proposed that this method could be used in conjunction with RAPD markers for applications such as genetic analysis, bulked segregant analysis, and quantitative trait loci mapping, especially in laboratories with a preference for agarose gel electrophoresis.
Abstract: Random amplified polymorphic DNA (RAPD) markers have been used for numerous applications in plant molecular genetics research despite having disadvantages of poor reproducibility and not generally being associated with gene regions. A novel method for generating plant DNA markers was developed based on the short conserved region flanking the ATG start codon in plant genes. This method uses single 18-mer primers in single primer polymerase chain reaction (PCR) and an annealing temperature of 50°C. PCR amplicons are resolved using standard agarose gel electrophoresis. This method was validated in rice using a genetically diverse set of genotypes and a backcross population. Reproducibility was evaluated by using duplicate samples and conducting PCR on different days. Start codon targeted (SCoT) markers were generally reproducible but exceptions indicated that primer length and annealing temperature are not the sole factors determining reproducibility. SCoT marker PCR amplification profiles indicated dominant marker like RAPD markers. We propose that this method could be used in conjunction with these markers for applications such as genetic analysis, bulked segregant analysis, and quantitative trait loci mapping, especially in laboratories with a preference for agarose gel electrophoresis.
841 citations
796 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 54 |
| 2024 | 157 |
| 2023 | 51 |
| 2022 | 67 |
| 2021 | 83 |
| 2020 | 90 |