TL;DR: A simple technique for consistent production of clean, unwrinkled, flat thin (500-1000 A) sections for TEM and thick sections for HVEM mounted on Formvar-covered slot grids for use in conventional and high voltage electron microscopy.
TL;DR: In this article, the authors compared cloud liquid water content derived from a formvar replicator, a Johnson-Williams probe and a forward scattering spectrometer probe (FSSP) in the presence of ice crystals.
Abstract: Airborne measurements of cloud liquid water content derived from a formvar replicator, a Johnson-Williams probe and a forward scattering spectrometer probe (FSSP) are compared. These show that in the presence of ice crystals the FSSP droplet spectra may be artificially enhanced. Typically the ice produces a flat distribution superimposed on the actual droplet distribution. The concentrations measured by the FSSP in the presence of ice are found to be 2–3 orders of magnitude greater than the actual ice concentrations as measured by the formvar replicator and a 2D-C probe. Possible explanations for the abnormal behavior of the FSSP in the presence of ice particles are discussed.
TL;DR: A method has been developed which makes it possible to obtain virtually wrinkle-free sections for electron microscopy bypicking up sections together with a drop of liquid in a one-hole grid which has no supporting film.
TL;DR: In this paper, a gas ionization counter is used as a thin (0.8 mg/cm2) transmission detector in a counter telescope particle-identification system, and the response of the system is approximately linear in Z for the ions produced by heavy ion-induced fission of medium-Z targets.
TL;DR: In this article, it has been shown that a palladium-platinum mixture is the most satisfactory material for use in the pre-shadowed replica technique, and that films of this mixture in a thickness of about 6A produce adequate shadows in which there is no sign of granulation.
Abstract: The lower limit of size of biological objects which can be photographed with the electron microscope, by the aid of shadow‐casting, is shown to depend upon the smoothness of substrate upon which they can be mounted, and upon the continuity of structure of the thin films with which they are shadowed. Numerous attempts to improve the existing deficiencies are reported, both with respect to producing smoother substrate films, and to producing films for shadow‐casting of high efficiency and continuity of structure. No success has been encountered in producing a usable substrate film perceptibly smoother than the collodion and Formvar films commonly used. It is found that the best shadow‐casting material for this type of film is uranium or uranium oxide. Verification has been obtained of the severe granulation of goldfilms previously used in the pre‐shadowed replica process, when subjected to the electron current of a biased‐beam electron gun. Attempts to reduce the granulation to a satisfactory level have failed. Various methods of preparing pre‐shadowed replicas are reported. The factors affecting the tenacity of evaporated films to glass surfaces are discussed. It is found that elements which oxidize readily are relatively adherent to glass, while the elements gold,palladium,platinum, and rhodium are not. Uranium sulfide can be used as a pre‐shadowed replica material, but only with some uncertainty, owing to its chemical instability. It has been found that a palladium‐platinum mixture is the most satisfactory material for use in the pre‐shadowed replica technique, and that films of this mixture in a thickness of about 6A produce adequate shadows in which there is no sign of granulation. The surface of clean glass is again found to have the smoothest structure of any material known, with practically no sharp discontinuities in elevation as great as 10A. An appendix is given, in which technical details of shadow‐casting and replica production are described.