Topic
Flaveria
About: Flaveria is a research topic. Over the lifetime, 194 publications have been published within this topic receiving 6474 citations.
Papers published on a yearly basis
Papers
TL;DR: Detailed promoter/reporter gene studies were performed in the closely related C4 species F. bidentis, revealing that the C4 promoter contains two regions, a proximal segment up to −570 and a distal part from −1566 to −2141, which are necessary but also sufficient for high mesophyll-specific expression of the β-glucuronidase reporter gene.
Abstract: C(4) photosynthesis depends on the strict compartmentalization of CO(2) assimilatory enzymes cis-regulatory mechanisms are described that ensure mesophyll-specific expression of the gene encoding the C(4) isoform of phosphoenolpyruvate carboxylase (ppcA1) of the C(4) dicot Flaveria trinervia To elucidate and understand the anatomy of the C(4) ppcA1 promoter, detailed promoter/reporter gene studies were performed in the closely related C(4) species F bidentis, revealing that the C(4) promoter contains two regions, a proximal segment up to -570 and a distal part from -1566 to -2141, which are necessary but also sufficient for high mesophyll-specific expression of the beta-glucuronidase reporter gene The distal region behaves as an enhancer-like expression module that can direct mesophyll-specific expression when inserted into the ppcA1 promoter of the C(3) plant F pringlei Mesophyll expression determinants were restricted to a 41-bp segment, referred to as mesophyll expression module 1 (Mem1) Evolutionary and functional studies identified the tetranucleotide sequence CACT as a key component of Mem1
259 citations
TL;DR: A comparative transcriptome analysis in leaves of five closely related C3, C4, and C3-C4 intermediate Flaveria species is carried out to gain insight into the extent to which gene expression patterns were altered in the evolutionary progression from C3 to C4.
Abstract: Selective pressure exerted by a massive decline in atmospheric CO(2) levels 55 to 40 million years ago promoted the evolution of a novel, highly efficient mode of photosynthetic carbon assimilation known as C(4) photosynthesis. C(4) species have concurrently evolved multiple times in a broad range of plant families, and this multiple and parallel evolution of the complex C(4) trait indicates a common underlying evolutionary mechanism that might be elucidated by comparative analyses of related C(3) and C(4) species. Here, we use mRNA-Seq analysis of five species within the genus Flaveria, ranging from C(3) to C(3)-C(4) intermediate to C(4) species, to quantify the differences in the transcriptomes of closely related plant species with varying degrees of C(4)-associated characteristics. Single gene analysis defines the C(4) cycle enzymes and transporters more precisely and provides new candidates for yet unknown functions as well as identifies C(4) associated pathways. Molecular evidence for a photorespiratory CO(2) pump prior to the establishment of the C(4) cycle-based CO(2) pump is provided. Cluster analysis defines the upper limit of C(4)-related gene expression changes in mature leaves of Flaveria as 3582 alterations.
210 citations
188 citations
TL;DR: Confinement of glycine decarboxylase to the bundle-sheath cells is likely to improve the potential for recapture ofPhotorespired CO2 via the Calvin cycle and could account for the low rate of photorespiration in all C3−C4 intermediate species.
Abstract: Immunogold labelling has been used to determine the cellular distribution of glycine decarboxylase in leaves of C3, C3−C4 intermediate and C4 species in the genera Moricandia, Panicum, Flaveria and Mollugo. In the C3 species Moricandia foleyi and Panicum laxum, glycine decarboxylase was present in the mitochondria of both mesophyll and bundle-sheath cells. However, in all the C3−C4 intermediate (M. arvensis var. garamatum, M. nitens, M. sinaica, M. spinosa, M. suffruticosa, P. milioides, Flaveria floridana, F. linearis, Mollugo verticillata) and C4 (P. prionitis, F. trinervia) species studied glycine decarboxylase was present in the mitochondria of only the bundle-sheath cells. The bundle-sheath cells of all the C3−C4 intermediate species have on their centripetal faces numerous mitochondria which are larger in profile area than those in mesophyll cells and are in close association with chloroplasts and peroxisomes. Confinement of glycine decarboxylase to the bundle-sheath cells is likely to improve the potential for recapture of photorespired CO2 via the Calvin cycle and could account for the low rate of photorespiration in all C3−C4 intermediate species.
157 citations
TL;DR: The most notable finding from these investigations was the identification of a C4 PEPC invariant site-specific mutation from alanine to serine at position 774 that was a necessary and late step in the evolution of C3 to C4PEPC.
143 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 4 |
| 2020 | 2 |
| 2019 | 2 |
| 2018 | 5 |
| 2017 | 3 |
| 2016 | 4 |