TL;DR: In this paper, the effects of a water-soluble fraction of used vehicle crankcase oils (WSF-UVCO) on the antioxidant responses of the flame scallop Ctenoides scaber were evaluated.
Abstract: Used vehicle crankcase oils are a source of contamination in Caribbean marine environments and may alter the oxidative balance of organism that inhabiting coastal ecosystems. This paper aims to evaluate effects of a water-soluble fraction of used vehicle crankcase oils (WSF-UVCO) on the antioxidant responses of the flame scallop Ctenoides scaber. The organisms were exposed to ascending sublethal concentrations 0, 0.001, 0.01 and 0.1 % of WSF-UVCO in a static system of aquaria during one week. Subsequently activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) as well as concentrations of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were determined in the digestive gland, adductor muscle and gills. SOD, CAT, GST and TBARS increased in digestive gland of organisms exposed to WSF-UVCO at medium and highest concentrations, with a concomitant decrease in GPX and GR activities. In adductor muscle CAT decreased, but GR rose with exposure to 0.01 and 0.1 % WSF-UVCO; in gills, GST rose through all WSF-UVCO concentrations, and SOD, CAT and GR increased only at 0.1 %. The fluctuations in antioxidant enzymes and GST activities point out possible adjustments to control ROS production and detoxification of xenobiotics. These biochemical responses may guarantee the oxidative balance in flame scallop during short term exposure to low concentrations of WSF-UVCO. C. scaber appears suitable as an experimental organism for evaluating biological risks of sublethal exposure to hazardous xenobiotics in tropical marine environments.
TL;DR: In this paper, morphological characters were compared to a molecular phylogeny constructed using three genes (COI, 28S, and H3) in order to differentiate Ctenoides scaber and C. mitis.
Abstract: One of the most well-known species in the bivalve family Limidae (d’Orbigny, 1846) is the brightly colored Ctenoides scaber (Born, 1778), commonly known as the rough file clam or flame scallop. Distinguishing this bivalve from its close relative, C. mitis (Lamarck, 1807), can be difficult using only morphological features and has led to much taxonomic confusion throughout the literature. In this study, morphological characters were compared to a molecular phylogeny constructed using three genes (COI, 28S, and H3) in order to differentiate C. scaber and C. mitis. The phylogeny recovered two well-supported clades that differ significantly in shell rib numbers, but not tentacle colors. The two species were then placed in a larger phylogenetic context of the Limidae family, which revealed the need for further systematic revision across genera. As these bivalves are popular in aquaria, cannot be tank-raised, and have been overcollected in the past, proper species identification is important for assessing sustainable collection practices.
TL;DR: The presence throughout the year of juveniles, ripe and partially spawned flame scallops and chlorophyll-a concentrations sufficient to support gamete development suggest a reproductive strategy of continuous spawning, common in tropical marine invertebrates.
Abstract: The reproductive cycle, sex distribution, and gonadal characteristics of the flame scallop, Ctenoides scaber, formerly Lima scabra scabra (Born 1778), collected from Boca Chica Key, FL, were investigated over the 21-mo period from January 1998 through September 1999. Gametogenic cycles were examined using qualitative and quantitative methods, and the relationships between those observations and environmental conditions (e.g., water temperature, salinity and phytoplankton concentrations) were analyzed. The relationships between sex, gonad color and shell height were also examined. Gamete development in both sexes was initiated in winter and was associated with small oocytes and follicles, cool water temperatures and moderate concentrations of food. Growth of gametes occurred throughout spring, as temperature and chlorophyll-a concentrations increased. A partial synchronous spawn occurred in early summer but did not seem to be related to environmental conditions. Maximum gamete ripeness and size oc...