TL;DR: Bioassay-directed fractionation of the CH2Cl2-MeOH extract of Euodia roxburghiana resulted in the isolation of two known quinoline alkaloids, buchapine and 2, and three new furoquinoline alkAloids, roxiamines A, B, and C (3-5).
Abstract: Bioassay-directed fractionation of the CH2Cl2-MeOH extract of Euodia roxburghiana resulted in the isolation of two known quinoline alkaloids, buchapine (1) and 2, and three new furoquinoline alkaloids, roxiamines A, B, and C (3-5). Compounds 1 and 2 protected CEM-SS cells from the cytopathic effects of HIV-1 in vitro (EC50 0.94 and 1.64 microM, respectively), but 3-5 were inactive against HIV-1.
TL;DR: Fla-vonoids isolated from Euodia daniellii exhibited no cytotoxic activity against the human tumor cell lines, A549, SKOV-3, SKMEL-2, XF498, and HCT15.
Abstract: Four flavonoid glycosides, flavaprin (7), evodioside B (8), vitexin (11), and hesperidin (12), as well as the coumarins bergapten (1), xanthotoxin (2), and isopimpinellin (3), the lignan simplex-oside (10), the steroids β-sitosterol (4) and daucosterol (5), the limonoids isolimonexic acid (6) and limonin (9), and uracil (13) andmyo-inositol (14) have been isolated fromEuodia daniellii. The structures of these compounds were established from spectral data. Among the isolates, bergapten showed cyclooxygenase-2 inhibitory activity with an IC50 value of 6.2 μg/ml. Fla-vonoids isolated from this plant exhibited no cytotoxic activity against the human tumor cell lines, A549, SKOV-3, SKMEL-2, XF498, and HCT15.
TL;DR: The inferred phylogeny lends support to merging these two subfamilies established by Engler based on different fruit types and shows that members of Euodia sensu lato should be placed in three different genera: Tetradium, EUodia, and Melicope.
Abstract: Plastid (trnL intron and trnL-F spacer) and nuclear (ITS-1 and ITS-2 rDNA) regions were analyzed to infer the phylogeny and evaluate the classification of Rutaceae subfamilies Rutoideae and Toddalioideae. The inferred phylogeny lends support to merging these two subfamilies established by Engler based on different fruit types. Moreover, Phellodendron, Tetradium, Toddalia, and Zanthoxylum were resolved as a clade, supporting the proposal for a 'proto-Rutaceae' group. The molecular data also showed that members of Euodia sensu lato should be placed in three different genera: Tetradium, Euodia, and Melicope. The latter two genera are more closely related to Acronychia than they are to Tetradium. Except for the discrepancy in the position of Melicope vitiflora, the cladistic results are congruent with the morphological and biochemical interpretations made by two previous authors.
TL;DR: The chemosystematic significance of the addition of Tetradium to the 1-benzyltetrahydroisoquinoline producing genera and the potential of these taxa to have acted as a starting point for biochemical evolution within the Rutaceae is discussed.
TL;DR: The results based on five plastid and nuclear markers have revealed that Acronychia, Euodia and Melicope are each not monophyletic in their current circumscriptions and that several small genera mainly from Australia and New Caledonia need to be merged with one of the three genera to ensure monophyly at the generic level.