TL;DR: All but two of the eight compounds tested were significantly different from the solvent controls in the filter paper consumption bioassay, resulting in the isolation of eight thiophenes possessing varying degrees of termiticidal activity.
Abstract: Over 220 crude extracts from repositories generated from plants native to Greece and Kazakhstan were evaluated for termiticidal activity against the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). Emerging from this screening effort were bioactive extracts from two Greek species (Echinops ritro L. and Echinops spinosissimus Turra subsp. spinosissimus) and extracts from two Kazakhstan species (Echinops albicaulis Kar. & Kir. and Echinops transiliensis Golosh.). Fractionation and isolation of constituents from the most active extracts from each of the four species has been completed, resulting in the isolation of eight thiophenes possessing varying degrees of termiticidal activity. 2,2':5',2"-Terthiophene and 5'-(3-buten-1-ynyl)-2,2'-bithiophene demonstrated 100% mortality against C. formosanus within 9 days at 1 and 2 wt% concentrations respectively. In addition, all but two of the eight compounds tested were significantly different from the solvent controls in the filter paper consumption bioassay.
TL;DR: The protoplast to plant regeneration protocols developed in this study may provide the basis to investigate cell physiology and biochemistry of desert medicinal plants.
TL;DR: A protocol for establishing nodal cultures of an Egyptian medicinal plant, Echinops spinosis-simus Turra and for the induction of regeneration in epidermal peels and leaf explants obtained from the nodal nations, finding Kinetin was found to be optimal for induction and proliferation of nodsal cultures.
Abstract: The in vitro propagation of medicinal species is proving to be one successful approach to addressing the issues raised by increasing global demand, overharvest of wild populations and variability in product quality. In this report we describe a protocol for establishing nodal cultures of an Egyptian medicinal plant, Echinops spinosis-simus Turra and for the induction of regeneration in epidermal peels and leaf explants obtained from the nodal cultures. Kinetin was found to be optimal for induction and proliferation of nodal cultures. Transverse slices of leaf blades produced regenerants resembling somatic embryos in the presence of thidiazuron while regeneration was observed on epidermal peels only when the medium contained elevated auxin concentrations.
TL;DR: In this article, a new isocomene sesquiterpenoid, 11-hydroxyisocom-2-en-5-one, was isolated from the roots of Echinops spinosissimus subsp. Greuter.
Abstract: A novel isocomene sesquiterpenoid, 11-hydroxyisocom-2-en-5-one, was isolated from the roots of Echinops spinosissimus subsp. spinosus Greuter. The structure was characterized on the basis of NMR spectroscopic methods, including 1H–1H COSY, HMBC, HSQC, and NOESY experiments.