Topic
dnaN
About: dnaN is a research topic. Over the lifetime, 366 publications have been published within this topic receiving 18899 citations.
Papers published on a yearly basis
Papers
TL;DR: The crystal structure of the processivity factor required by eukaryotic DNA polymerase delta, proliferating cell nuclear antigen from S. cerevisiae, has been determined and the dimensions and electrostatic properties of the ring suggest that PCNA encircles duplex DNA, providing a DNA-bound platform for the attachment of the polymerase.
952 citations
TL;DR: The crystal structure of the human DNA polymerase delta processivity factor PCNA (proliferating cell nuclear antigen) complexed with a 22 residue peptide derived from the C-terminus of the cell-cycle checkpoint protein p21(WAF1/CIP1) has been determined at 2.6 angstrom resolution.
834 citations
TL;DR: A potential structural relationship is suggested between the beta subunit and proliferating cell nuclear antigen (PCNA, the eukaryotic polymerase delta [and epsilon] processivity factor), and the gene 45 protein of the bacteriophage T4 DNA polymerase.
785 citations
TL;DR: A heteropentameric AAA+ clamp-loading machine that couples ATP hydrolysis to load circular clamp proteins onto DNA and twin polymerases and clamps coordinate their actions to form a replisome machine that advances the replication fork.
Abstract: ▪ Abstract Chromosomal DNA replicases are multicomponent machines that have evolved clever strategies to perform their function. Although the structure of DNA is elegant in its simplicity, the job of duplicating it is far from simple. At the heart of the replicase machinery is a heteropentameric AAA+ clamp-loading machine that couples ATP hydrolysis to load circular clamp proteins onto DNA. The clamps encircle DNA and hold polymerases to the template for processive action. Clamp-loader and sliding clamp structures have been solved in both prokaryotic and eukaryotic systems. The heteropentameric clamp loaders are circular oligomers, reflecting the circular shape of their respective clamp substrates. Clamps and clamp loaders also function in other DNA metabolic processes, including repair, checkpoint mechanisms, and cell cycle progression. Twin polymerases and clamps coordinate their actions with a clamp loader and yet other proteins to form a replisome machine that advances the replication fork.
616 citations
TL;DR: Bacteriophage T7 gene 5 protein has been purified to apparent homogeneity from cells overexpressing its gene several hundred-fold, and DNA synthesis catalyzed by the gene 5protein-thioredoxin complex on a single-stranded DNA template can polymerize thousands of nucleotides without dissociation.
433 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2022 | 1 |
| 2021 | 15 |
| 2020 | 7 |
| 2019 | 13 |
| 2018 | 18 |
| 2017 | 23 |