Topic
Diplotaxis muralis
About: Diplotaxis muralis is a research topic. Over the lifetime, 32 publications have been published within this topic receiving 521 citations.
Papers
TL;DR: The genetic relationships among the ten Diplotaxis species estimated by the polymorphism of ISSR markers are in agreement with those previously inferred by other morphological, biochemical and molecular data, indicating the reliability of the ISSR approach for this purpose.
Abstract: Inter-simple sequence repeat (ISSR) amplification was evaluated for its applicability as a genetic marker system to establish relationships among ten Diplotaxis species. ISSR amplification generated multiple banding profiles with the 12 primers from all DNA samples, with an average of 41.2 fragments per primer. This average was clearly higher for the 5´ triple-anchored primers than for other primers. The banding profiles were highly repeatable across separate PCR runs. DNA mixing procedures were found to be appropriate strategies to generate banding patterns representative of each species studied. Similarity values were calculated considering 494 ISSR bands, and a dendrogram was constructed based on the similarity matrix. The ten Diplotaxis species were clustered into two major groups. The first group consists of five species, Diplotaxis tenuifolia and Diplotaxis cretacea, and Diplotaxis muralis with their putative parents (D. tenuifolia and Diplotaxis viminea). In the second group three species are clustered that are closely related (Diplotaxis virgata, Diplotaxis catholica and Diplotaxis siettiana), in addition to Diplotaxis harra, and Diplotaxis erucoides, which has lowest similarity values with the rest of the species studied. The two groups defined in the present work may be concordant with the idea suggested by several authors of a biphyletic origin for Diplotaxis. The genetic relationships among the ten Diplotaxis species estimated by the polymorphism of ISSR markers are in agreement with those previously inferred by other morphological, biochemical and molecular data, indicating the reliability of the ISSR approach for this purpose.
96 citations
TL;DR: Two types 0L male sterility were recognized in the strain; one was completely male sterile (MS) without anther dehiscence and the other was partiallymale sterile (PMS), with a little emission of pollen.
Abstract: 1. D. muralis was used as a female parent in crosses with B. campestris (a commercial cultivar, Yukina). Successive backcrossing by the pollen of the latter species raised a male sterile strain having Yukina nucleus and D. muralis cytoplasm. 2. Morphological characters 0L this strain were quite alike to cv. Yukina, except flower organs; such as two nectaries, narrow petals and short filaments with stunted anthers in which was observed a small quantity of pollen. Pistils were normal in mor-phology as well as in function. 3. Two types 0L male sterility were recognized in the strain; one was completely male sterile (MS) without anther dehiscence and the other was partially male sterile (PMS), with a little emission of pollen. In order to obtain a family whose plants are all MS, maintainers were made by two methods, from a selfed progeny of the cultivar and from a cross Yukina>
74 citations
TL;DR: The primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled and it was realised that true somatic hybrids and partial Somatic hybrids had been generated.
Abstract: The need to transfer genetic traits from Diplotaxis muralis (2n=42) to Brassica juncea (2n=36), a major oil seed crop of the tropical world, was realised. Since the two plant types are sexually incompatible, attempts were made to evolve parasexual hybrids as the result of protoplast fusion. Protoplasts of hypocotyl-derived calli of two cultivars of B. juncea were fused with normal and γ-irradiated mesophyll protoplasts of Diplotaxis muralis. Regeneration of 110 plants from the fused products was successfully achieved. Upon analysis of some of them, we realised that true somatic hybrids and partial somatic hybrids had been generated. Thus the primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled.
47 citations
TL;DR: A CMS-associated chimeric mitochondrial gene, orf72, was found in the mitochondrial genome of donor cytoplasm and was located downstream of normal rps7 and contained part of atp9 (atp9-b).
Abstract: Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male-sterile (CMS) plants caused by disturbed mitochondrial-nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the 'mur' system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.
35 citations
TL;DR: F1 plants obtained from crosses with the B. napus cultivars ‘Mangum’ and ‘Hinchu’ were male-sterile while F1 plants derived from all other crosses weremale-fertile, indicating that these two cultivars carried maintainer genes far themale-sterility-inducing cytoplasm of D. muralis.
Abstract: Male fertility of F1 interspecific hybrid plants derived from crosses between cytoplasmic male-sterile Brassica campestris in Diplotaxis muralis cytoplasm and 147 B. napus cultivars was Investigated. F1, plants obtained, from crosses with the B. napus cultivars‘Mangum’and‘Hinchu’were male-sterile while F1 plants derived from all other crosses were male-fertile. This indicated that these two cultivars carried maintainer genes far the male-sterility-inducing cytoplasm of D. muralis. Sterility was stable In plants derived from backcrosses of male-sterile F; plants with‘Mangun and‘Hinchu’but the seed set of backcross plants was low. With restorer genes readily available in B. napus, these findings could lead to the development of a new cytoplasmic male sterility system for the breeding of B. napus hybrid cultivars.
28 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2017 | 2 |
| 2012 | 1 |
| 2009 | 1 |
| 2006 | 2 |
| 2004 | 2 |
| 2003 | 2 |