TL;DR: ITS-2 ribosomal DNA analyses showed that this molecule is well suited for resolving evolutionary relationships at various taxo-nomic levels, and it is proposed to regard both groups as distinct genera, Desmodesmus gen. nov. and Scenedesmus sensu strictu, and to merge Acutodesmus into Sceningesmus.
Abstract: Analyses of ITS-2 ribosomal DNA from Scenedesmus and Scenedesmus-like strains showed that this molecule is well suited for resolving evolutionary relationships at various taxo-nomic levels. The analyses clearly suggested the separation of the investigated strains into two groups which corresponded to the “subgenera”Desmodesmus and Scenedesmus/Acutodesmus. No clear distinction between Acutodesmus and Scenedesmus was seen. It is proposed to regard both groups as distinct genera, Desmodesmus gen. nov. and Scenedesmus sensu strictu, and to merge Acutodesmus into Scenedesmus. Monophyly of both genera, Scenedesmus and Desmodesmus, is further supported by certain secondary structure features of ITS-2. The relationships resolved by the ITS-2 analyses are congruent with findings from cell wall ultrastructure, but not with those from other morphological features such as cell wall surface structures or cell shape. Didymocystis inermis and Dicloster acuatus, though somewhat similar in vegetative cell morphology to species of Scenedesmus and Desmodesmus, were seen as members of independent genera because of substantial differences in primary structure and length variations in ITS-2 when compared with each other and the latter two genera.
TL;DR: A survey on the Scenedesmaceae from 10 fishing ponds in Sao Paulo Metropolitan Region (RMSP) was performed, and Scenedesmus and Desmodesmus are the best represented genera, with nine and eight species, respectively.
Abstract: A survey on the Scenedesmaceae from 10 fishing ponds in Sao Paulo Metropolitan Region (RMSP) was performed. The samples were collected from the sub-surface, horizontally towed with a plankton net (20 µm mesh), in two periods: September-October 2001 (dry season) and February-March 2002 (rainy season), with a total of 20 samples. The samples were preserved in 4-5% formaldehyde solution, and studied under a photonic microscope. Twenty-six taxa of Scenedesmaceae were identified, distributed in 10 genera, 23 species, two varieties and one taxonomic form. Desmodesmus lefevrei (Deflandre) An, Friedl & Hegewald, Dicloster acuatus Jao, Wei & Hu and Scenedesmus baculiformis Chodat are cited for the first time for the State of Sao Paulo. Scenedesmus and Desmodesmus are the best represented genera, with nine and eight species, respectively.
TL;DR: According to the results a subfamily Desmodesmoideae was set up, the subgenera Acutodesmus and Scenedesmus were verified and Scensesmus (Kermatia) pupukensis was transferred to Neodesmus.
Abstract: 18S-RNA was analysed for 17 species from 8 genera of Scenedesmaceae sensu KOMAREK et FOTT (1983) and one strain of Coelastropsis (Coelastraceae) and Coelastrella (Scotiellaceae). According to the results a subfamily Desmodesmoideae was set up, the subgenera Acutodesmus and Scenedesmus were verified and Scenedesmus (Kermatia) pupukensis was transferred to Neodesmus. Dactylococcus dissociatus and the genus Tetradesmus were included in Acutodesmus. The two strains of Tetradesmus as well as the two strains of the varieties of S. pectinatus were not closely related and the placement of S. pectinatus var. pectinatus was not stable. It probably belongs to Enallax, while the other taxa belong to Acutodesmus, A strain labelled Schroederiella apiculata was recognised as a new species of Scenedesmus (S. hindakii). Scenedesmus acutiformis clustered separately and is preliminarily accepted as Enailax acutiformis. Pseudodidymocystis is a sister group to Desmodesmus. Dicloster was excluded from Scenedesmaceae and also from Chlorophyceae and is placed in Trebouxiophyceae. Coelastropsis costata has a 18S similar to Scenedesmus s.str., but is morphologically not closely related to that genus and is hence not transferred to Scenedesmus. The former Chlorella fusca var. vacuolatus (=Scenedesmus vacuolatus) is transferred to Coelastrella.