Showing papers on "Derivatization published in 1972"
TL;DR: This chapter presents gas-liquid chromatography methods for the separation of D and L amino acids in the Murchison meteorite, and it seems that the GLC method has its greatest applicability in this area of analysis.
Abstract: Publisher Summary This chapter presents gas-liquid chromatography (GLC) methods for the separation of D and L amino acids. An interesting application is the analysis of amino acids in the Murchison meteorite. The D and T, enantiomers of certain amino acids are determined as the N-TFA- D -2-butyl esters. Optically active stationary phases are used to separate stereoisomers of amino acids. In considering the relative merits of the GLC method to established ion-exchange procedures, an important factor is the sample preparation prior to derivatization. Most biological samples require a separation of amino acids from interfering substances on a cation-exchange resin. Some samples, such as urine, require a clean-up on both cation and anion exchange resins. The samples could be analyzed directly by the established ion-exchange methods and in less time than it takes to accomplish the clean-up on both cation and anion exchange resins. Clean-up of hydrolyzates of most pure proteins and peptides is not necessary, and it seems that the GLC method has its greatest applicability in this area of analysis.
34 citations
TL;DR: The molar responses of trifluoroacetylated amino acid methyl esters were determined with the flame ionization detector and non-linearity of response could be used to show either incomplete derivatization or breakdown of the derivative during gas chromatography.
28 citations
TL;DR: A gas chromatographic assay for melatonin based on the formation of a diheptafluorobutyrate that permits the quantitative determination of melatonin in rat pineals and assures that at least the quantitated amount is present.
26 citations
17 citations
13 citations
TL;DR: Specific heavy atom derivatization of valyl and arginyl tRNA's from E. coli has been effected by the use of the N-hydroxysuccinimide esters of certain carboxylic acids and are shown to be stable under the conditions required for crystallization.
12 citations
TL;DR: The lability of various silylated and acetylated derivatives of the tertiary aminopropiophenones of eugenol was characterized by combined gas—liquid chromatography and chemical ionization mass spectrometry to find “d”-silyl ethers which can be satisfactorily characterized by gas chromatography.
9 citations
TL;DR: The optical purity of 19 labeled amino acids was determined by thin-layer chromatography of dipeptides formed by derivatization with l -leucine- N -carboxyanhydride.
9 citations
TL;DR: A rapid, accurate, and precise gas chromatographic method for the determination of lysine in acid hydrolyzates of wheat and rice seed appears applicable to many types of seeds with suitable adaptations to specific needs.
8 citations
TL;DR: UV, IR, and NMR spectra of the purified concentrates confirmed the derivatization of the parent compounds and could be helpful in the identification of these compounds and in determining the structure of closely related compounds isolated from biological samples.
5 citations
TL;DR: Citric and nitrilotriacetic acids can be determined at the 1–10,000 p.p.b. 1 levels in aqueous systems ranging from tap water to sewage effluents by use of anion-exchange clean-up, derivatization with butanol—HCl and gas chromatography.