Showing papers on "Derivatization published in 1971"
TL;DR: The data for amino acids in ribonuclease, β-casein, K- casein, soybean meal, and blood are in good agreement with the values obtained by the classical ion-exchange method, and establishes the use of the trimethylsilyl/gas—liquid chromatographic method for the quantitative analysis of biological materials.
188 citations
TL;DR: The broad spectrum of application for this technique has already included the search for amino acids in the Returned Lunar Samples, and would range from studies at the cellular level, investigations on the molecular basis of disease, to the analysis for trace amounts of environmental pollutants.
71 citations
TL;DR: In this paper, a general procedure for the N-acetylation and N,O,S-permethylation of unknown peptides that do not contain arginine is described.
Abstract: The amino acid sequence of oligopeptides can be determined from the mass spectra of their volatile derivatives. A general procedure for the N-acetylation and N,O,S-permethylation of unknown peptides that do not contain arginine is described. The procedure is illustrated by the derivatization of lysyl-tyrosyl-glutamic acid. The mass spectrum of the resulting N a, N' -diacetyl-N a, N'-dimethyllysyl-N a, O-dimethyltyrosyl-Na-methyl glutamic acid α, β dimethyl ester (I)1 is given.
63 citations
24 citations
TL;DR: A one-step procedure for the simultaneous derivatization of acidic, neutral and basic metabolites of tyrosine and tryptophan has been developed and the derivatives were quantitatively determined by gas-liquid chromatography using three stationary phases of varying selectivities.
20 citations