Topic
Derivatization
About: Derivatization is a research topic. Over the lifetime, 11408 publications have been published within this topic receiving 261774 citations.
Papers published on a yearly basis
1 / 2
Papers
TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
2,543 citations
TL;DR: All amino acids, including proline, are converted quantitatively to phenylthiocarbamyl compounds and these are stable enough to eliminate any need for in-line derivatization, providing results comparable in sensitivity and precision to those obtained by state-of-the-art ion-exchange analyzers.
1,625 citations
TL;DR: Analysis of fatty acid methyl esters prepared from whole-cell bacteria by sodium hydroxide hydrolysis and boron trichloride-catalyzed methylation showed degradation of hydroxy acid peaks after several injections on a fused silica capillary column, eliminating the need to form trifluoroacetic anhydride derivatives of the hydroxy acids.
Abstract: Analysis of fatty acid methyl esters prepared from whole-cell bacteria by sodium hydroxide hydrolysis and boron trichloride-catalyzed methylation showed degradation of hydroxy acid peaks after several injections on a fused silica capillary column. A simple base wash of the extracts before injection prevented the tailing of the hydroxy acid peaks even after extended use. This eliminates the need to form trifluoroacetic anhydride derivatives of the hydroxy acids.
1,086 citations
TL;DR: A highly reactive amine derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, has been synthesized in a rapid, one-step procedure to form stable unsymmetric urea derivatives which are readily amenable to analysis by reversed phase HPLC.
988 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 162 |
| 2024 | 344 |
| 2023 | 544 |
| 2022 | 890 |
| 2021 | 283 |
| 2020 | 308 |