Topic
Deoxyribose
About: Deoxyribose is a research topic. Over the lifetime, 1309 publications have been published within this topic receiving 51196 citations. The topic is also known as: 2-deoxy-D-ribose & 2-deoxy-D-erythro-pentose.
Papers published on a yearly basis
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Papers
TL;DR: A structure for nucleic acid has already been proposed by Pauling and Corey and it is believed that the material which gives the X-ray diagrams is the salt, not the free acid, so without the acidic hydrogen atoms it is not clear what forces would hold the structure together.
Abstract: A structure for nucleic acid has already been proposed by Pauling and Corey [1]. They kindly made'their manuscript available to us in advance of publication. Their model consists of three inter-twined chains, with the phosphates near the fibre axis, and the bases on the outside. In our opinion, this structure is unsatisfactory for two reasons: (1) We believe that the material which gives the X-ray diagrams is the salt, not the free acid. Without the acidic hydrogen atoms it is not clear what forces would hold the structure together, especially as the negatively charged phosphates near the axis will repel each other. (2) Some of the van der Waals distances appear to be too small.
2,755 citations
TL;DR: It is suggested that the deoxyribose assay is a simple and cheap alternative to pulse radiolysis for determination of rate constants for reaction of most biological molecules with hydroxyl radicals.
2,497 citations
869 citations
TL;DR: The role of this radical @ iron-dependent damage to the sugar deoxyribose is examined, showing that treatment of several amino acids or carbohydrates with iron (II) salts in phosphate buffer causes formation of a product that reacts with thiobarbituric acid (TBA) to produce a chromogen.
838 citations
TL;DR: The structural basis of the reaction of the hydroxyl radical with DNA is revealed, providing information on the mechanism of DNA damage caused by ionizing radiation as well as atomic-level detail for the interpretation of hydroxy radical footprints of DNA-protein complexes and chemical probe experiments on the structure of RNA and DNA in solution.
Abstract: Despite extensive study, there is little experimental information available as to which of the deoxyribose hydrogen atoms of duplex DNA reacts most with the hydroxyl radical. To investigate this question, we prepared a set of double-stranded DNA molecules in which deuterium had been incorporated specifically at each position in the deoxyribose of one of the four nucleotides. We then measured deuterium kinetic isotope effects on the rate of cleavage of DNA by the hydroxyl radical. These experiments demonstrate that the hydroxyl radical reacts with the various hydrogen atoms of the deoxyribose in the order 5′ H > 4′ H > 3′ H ≈ 2′ H ≈ 1′ H. This order of reactivity parallels the exposure to solvent of the deoxyribose hydrogens. Our work therefore reveals the structural basis of the reaction of the hydroxyl radical with DNA. These results also provide information on the mechanism of DNA damage caused by ionizing radiation as well as atomic-level detail for the interpretation of hydroxyl radical footprints of DNA-protein complexes and chemical probe experiments on the structure of RNA and DNA in solution.
559 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 12 |
| 2024 | 4 |
| 2023 | 17 |
| 2022 | 27 |
| 2021 | 9 |
| 2020 | 15 |