TL;DR: The Indochinese box turtle Cuora galbinifrons is a polytypic, critically endangered species from Vietnam, Laos, and Hainan Island, China as discussed by the authors.
TL;DR: The results of nucleotide base compositions, protein-coding genes and phylogenetic analysis showed that C. trifasciata from Vietnam and China formed separate monophyletic clades with different Cuora species.
TL;DR: The results confirm the controversial species and subspecies/morphotypes of the critically endangered Asian box turtle genus Cuora to be genetically distinct, which has critical implications for conservation strategies.
Abstract: We isolated and characterized 16 new di- and tetranudeotide microsatellite markers for the critically endangered Asian box turtle genus Cuora, focusing on the "Cuora trifasciata" species complex. The new markers were then used to analyse genetic variability and divergence amongst five described species within this complex, namely C. aurocapitata (n = 18), C. cyclornata (n = 31), C. pani (n = 6), C. trifasciata (n = 58), and C. zhoui (n = 7). Our results support the view that all five species represent valid taxa. Within two species (C. trifasciata and C. cyclornata), two distinct morphotypes were corroborated by microsatellite divergence. For three individuals, morphologically identified as being of hybrid origin, the hybrid status was confirmed by our genetic analysis. Our results confirm the controversial species (Cuora aurocapitata, C. cyclornata) and subspecies/morphotypes (C. cyclornata meieri, C. trifasciata cf. trifasciata) to be genetically distinct, which has critical implications for conservation strategies.
TL;DR: In this paper, a method for the preparation of cuora aurocapitata gelatin is described, in which the nutritional and healthcare components of the gelatin are left to the maximal extent.
Abstract: The invention discloses a preparation method of cuora aurocapitata gelatin. The method comprises the following steps: killing cuora aurocapitata, blanching to deodorize, slightly boiling and cutting,removing oil by strong fire, removing burrs by medium fire, primarily decocting and taking soup, secondarily decocting and taking juice, concentrating to obtain a concentrated soap, mixing juice, prepared auxiliary materials, main materials and auxiliary materials, drying and fishing to take gel, cooling, condensing, forming, dewatering, baking and the like; according to the prepared cuora aurocapitata gelatin, nutritional and healthcare components of the cuora aurocapitata are remained; the prepared cuora aurocapitata gelatin is high in solubility; the original nutritional components are remained to the maximal extent; the cuora aurocapitata gelatin is easy to absorb and is convenient to eat.