Topic
Creatinase activity
About: Creatinase activity is a research topic. Over the lifetime, 9 publications have been published within this topic receiving 94 citations.
Papers
TL;DR: Inhibition experiments suggested that a sulfhydryl group is closely related to the creatinase activity and was most active at pH 8 and showed a K m value of 1.33 m m for creatine.
33 citations
TL;DR: An unexpected finding was that both LKR and SDH were dual co-factor enzymes and not specific for either NAD(+) or NADP(+), as is the case in other organisms, suggesting it could be a good candidate for anthelmintic targeting.
21 citations
TL;DR: A genomic library of Pseudomonas putida DNA was constructed by using plasmid pBR322 and a unique protein band (with a molecular weight of approximately 50,000) was observed in recombinant E. coli by minicell analysis.
Abstract: A genomic library of Pseudomonas putida DNA was constructed by using plasmid pBR322. Transformants of Escherichia coli in combination with Proteus mirabilis cells grown on creatinase test plates were screened for creatinase activity; transformants were considered positive for creatinase activity if a red-pink zone appeared around the colonies. One creatinase-positive clone was further analyzed, and the gene was reduced to a 2.7-kb DNA fragment. A unique protein band (with a molecular weight of approximately 50,000) was observed in recombinant E. coli by minicell analysis.
13 citations
Journal Article•
12 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2012 | 1 |
| 2008 | 1 |
| 2003 | 1 |
| 1998 | 1 |
| 1993 | 1 |
| 1992 | 1 |