Topic
Corrosion Casting
About: Corrosion Casting is a research topic. Over the lifetime, 77 publications have been published within this topic receiving 1868 citations.
Papers published on a yearly basis
Papers
Journal Article•
TL;DR: In this paper, the main steps leading to the technique of scanning electron microscopy (SEM) of vascular corrosion casts are discussed and a detailed discussion of the physical and chemical properties of the casting media and their significance for polymerization, shrinkage, casting quality, corrosion resistance, and thermal and spatial stability is given.
Abstract: The present paper states very briefly the main steps leading to the technique of scanning electron microscopy (SEM) of vascular corrosion casts. From the terms presently used (injection method, microcorrosion cast, injection replica, vascular corrosion cast, vascular cast) the use of "vascular corrosion cast" for lymphatic and blood vessels is recommended. Specification and pretreatment (kind, volume, dosage of anticoagulants, vasoactive substances and spasmolytica used) of the animals examined are referenced as they are available from the literature. The recommendation is given to pay more attention to these parameters than done so far. The steps necessary for producing reasonable and suitable vascular corrosion casts are critically described. Special attention is paid to the physical and chemical properties of the casting media and their significance for polymerization, shrinkage, casting quality, corrosion resistance, and thermal and spatial stability. Emphasis is also focused on the advantages of cutting the vascular corrosion casts embedded in an ice block by a band saw, a self constructed multi-blade cutting device or a mini wheel-saw placed in the chamber of a cryomicrotome. From the drying methods presently used freeze-drying is stressed because of minimal specimen damage. To render casts conductive in most cases sputter-coating is sufficient. It is recommended to run the SEM with 5-10 kV since the resolution received still reveals all details the casting media presently can replicate. Further the application of scanning electron microscopy of vascular corrosion casts in fully differentiated normal tissue, in pathologic tissue as well as in developing tissues and organs is stated. Lastly possibilities and conditions are discussed under which SEM of vascular corrosion casts can serve to quantify vascular structures in order to make the technique more than pure descriptive.
269 citations
TL;DR: A new polyurethane‐based casting resin (PU4ii†) with superior physical and imaging characteristics is reported here about, which has great potential to support the description of vascular defects and drug effects in disease models using mutant mice.
Abstract: Vascular corrosion casting has been established as a method to reconstruct the three-dimensional (3D) structure of blood vessels of organs and tissues. After replacing the blood volume with a low viscosity resin, the surrounding tissue is removed to replicate the vascular architecture, typically using scanning electron microscopy (SEM). To date available casting resins have had significant limitations such as lack of viscosity, leading to insufficient perfusion of smallest capillaries in organs and tissues of smaller species, interaction with surrounding tissue or fragility of resulting casts. We have reported here about a new polyurethane-based casting resin (PU4ii) with superior physical and imaging characteristics. Low viscosity, timely polymerization, and minimal shrinking of PU4ii produces high quality casts, including the finest capillaries. These casts are highly elastic while retaining their original structure to facilitate postcasting tissue dissection and pruning of casts. SEM images illustrate the high reproduction quality, including endothelial cell imprints, features that allow one to discern arterial and veinal vessels. For quantitative analysis, casts from PU4ii can be imaged using micro-computed tomography to produce digital 3D reconstructions. The inherent fluorescence of PU4ii is sufficient to reproduce casts with or without tissue using confocal microscopy (CM). Because of the simplified casting procedure, the high reproducibility and the superior reproduction quality, a combination of vascular corrosion casting using PU4ii with advanced imaging technologies has great potential to support the description of vascular defects and drug effects in disease models using mutant mice.
147 citations
Journal Article•
129 citations
1 Jan 1992
96 citations
Journal Article•
TL;DR: One of the most important potential applications of the corrosion casting technique lies in the investigation of gastrointestinal, renal or hepatic ailments, which coincide with the reconstruction or rarefication of the vascular bed, e.g., in ulcers, ileitis terminalis, colitis ulcerosa, cirrhosis or glomerulonephritis.
Abstract: The aims of this review are: 1. to provide a bibliography of the publications that have used the corrosion casting technique; 2. to describe the advantages and limitations of the methodology; 3. to illustrate possible applications in the field of medicine, and 4. to highlight the significance of this method in the teaching of medical students. Thus, this paper is primarily focused on the scanning electron microscopical examination of vascular corrosion casts. The unsurpassed three-dimensionality of the corrosion casting technique compared to any other means stands out in particular. This can be especially useful when complex vascular-anatomical relationships are present. This applies not only to the portrayal of the modes of branching and varying vascular densities but also to regulatory arrangements, such as sphincters and arteriovenous anastomoses. Between 1966 and 1990, a total of 549 publications were found in the Medline literature data bank, containing the key words "corrosion casting", "microvascular cast", or "vascular cast" (as of August, 1990). Of those publications, most dealt with applications to experimental animals. By contrast, only 142 reports were mainly or partially concerned with human investigational material. The normal vascular system of nearly all organs, insofar as this is of direct medical relevance, has been largely resolved. In our opinion, one of the most important potential applications of the corrosion casting technique lies in the investigation of gastrointestinal, renal or hepatic ailments, which coincide with the reconstruction or rarefication of the vascular bed, e.g., in ulcers, ileitis terminalis, colitis ulcerosa, cirrhosis or glomerulonephritis.
83 citations
Related Topics (5)
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| 2022 | 1 |
| 2019 | 2 |
| 2017 | 2 |
| 2016 | 1 |
| 2015 | 2 |
| 2014 | 1 |