TL;DR: The dry seed coat cross section and surface structure of P. coronopus seeds before the first wetting were examined, as were the freeze-dried mucilage and adhesion of the seeds to the soil crust, which confirmed the importance of mucilaginous seeds for the survival of the plant species under desert conditions.
Abstract: Plantago coronopus var. crassipes is a common annual plant in many areas of the Negev Desert highlands. The dry seed coat cross section and surface structure of P. coronopus seeds before the first wetting were examined by SEM, as were the freeze-dried mucilage and adhesion of the seeds to the soil crust. When wetted, the mucilaginous layer forms, and seeds on the soil surface adhere to the soil crust. Some P. coronopus mucilaginous seeds may be dispersed by rain, even after less then 10 min of wetting. The dry seeds can float on run-on water for 10–44 min and are then dispersed along runnels into depressions, or adhere within a few minutes to the wet soil crust near the dry mother plant. Seeds matured on dry plants in a natural habitat in spring 1995, germinated in December 1995 to 96% after 72 h at 20 °C in light, but only to 2% in dark. The importance of mucilaginous seeds for the survival of the plant species under desert conditions is discussed.
TL;DR: From the population structure analyses, little gene flow in the species P. major and P. coronopus could be concluded, whereas P. lanceolata showed relatively high levels of gene flow, and the degree of homozygosity in the latter species was too high to explain with the available data.
Abstract: SummaryPopulations of the three Plantago species P. major, P. lanceolata and P. coronopus were scored for allozyme variability. They showed normal variability levels compared with other, similar plant species. Differentiation among populations appeared to be rather low in comparison with other species, probably due to a considerable amount of long distance seed transport. In order to be able to make an analysis of small-scale gene flow, all (sub)populations were critically checked for the existence of population structure equilibrium. The allozyme variation was tested for neutrality by testing homogeneity of F values among loci: between populations (Lewontin-Krakauer test) and within populations. No systematic deviations from the prediction of the neutral theory could be established. From the population structure analyses, little gene flow in the species P. major (with high selfing levels) and P. coronopus could be concluded, whereas P. lanceolata showed relatively high levels of gene flow. The degree of homozygosity in the latter species was too high to explain with the available data. In P. coronopus, on the other hand, an unusually high number of heterozygotes were observed.
TL;DR: High germination of both subspecies of P. lanceolata and P. coronopus seedlings showed opposite mortality curves and Root penetration of the surface occurred sooner on substrates of large particles than on those of small ones, in relation to the age of dry-stored seeds.
Abstract: SUMMARY
(a) The germination of Plantago lanceolata L., P. coronopus L., P. major L. ssp.major, P. major L. ssp. pleiosperma Pilger, P. media L., and P. maritima L. in relation to the age of dry-stored seeds was investigated under laboratory conditions. In general, higher percentages of radicle emergence were found with two- to five-year-old seed than with fresh seed.
Only P. lanceolata and P. media seeds germinated in complete darkness. A feature of interest was the high germination of both subspecies of P. major in so-called green safelight.
(b) The germination and early establishment were tested on various microsites, obtained with glass beads of various sizes. At increasing particle size, an increase in radicle emergence was found for large seeds (P. lanceolata and P. media). Root penetration of the surface occurred sooner on substrates of large particles than on those of small ones.
The effects of size of the glass beads on germination and penetration were less in species with small seeds than in species with large seeds.
(c) Sowing tests in the field resulted in relatively high numbers of established P. lanceolata and P. coronopus seedlings in open and dry habitats. P. lanceolata seedlings were able to establish themselves in tall dense vegetation layers. P. major ssp. major occurred most frequently on moist and open sites with a compact soil. On trodden paths P. major and P. coronopus seedlings showed opposite mortality curves.
TL;DR: In comparing the species, it appeared that P. coronopus and P. major showed good concordance in the distribution of both kinds of variability, each having only a slightly higher morphological than electrophoretic differentiation between populations.
Abstract: The level of electrophoretic variability in three Plantago species, P. major, P. coronopus, and P. lanceolata, was analyzed in relation to their breeding systems and compared with their morphological variability. From each species several populations were analyzed. The outcrossing P. lanceolata had the highest level of electrophoretic variability and the lowest population differentiation. The inbreeding P. major showed the opposite: a low level of electrophoretic variability and a high population differentiation. P. coronopus, with an intermediate breeding system, had an intermediate level of variability and differentiation. In comparing the species, it appeared that P. coronopus and P. major showed good concordance in the distribution of both kinds of variability, each having only a slightly higher morphological than electrophoretic differentiation between populations. P. lanceolata showed a higher morphological than electrophoretic differentiation between populations. A comparison of populations, within species, revealed good concordance of electrophoretic and morphological variability only within P. coronopus, while some populations of the other two species had relatively lower morphological variability compared with electrophoretic variability.
TL;DR: All isolates of S. minor obtained from the weed species were pathogenic to peanut and Koch's postulates were fulfilled to confirm pathogenicity of Sclerotinia minor on nine weed species.
Abstract: Bleached stems and sclerotia were observed on winter annual weed species growing in harvested peanut fields in northeastern North Carolina in March 2001. Each field had a history of Sclerotinia blight caused by Sclerotinia minor. Symptomatic plants were collected and brought back to the laboratory for identification and isolation. S. minor was isolated and Koch's postulates were fulfilled to confirm pathogenicity of S. minor on nine weed species. They included Lamium aplexicaule (henbit), Cardamine parviflora (smallflowered bittercress), Stellaria media (common chickweed), Cerastium vulgatum (mouse-ear chickweed), Coronopus didymus (swinecress), Oenothera laciniata (cutleaf eveningprimrose), Conyza canadensis (horseweed), Brassica kaber (wild mustard), and Arabidopsis thaliana (mouse-ear cress). This is the first report of these species as hosts of S. minor in the natural environment. All isolates of S. minor obtained from the weed species were pathogenic to peanut.