Topic
Coombs test
About: Coombs test is a research topic. Over the lifetime, 1120 publications have been published within this topic receiving 18971 citations. The topic is also known as: Coombs' test & antiglobulin test.
Papers published on a yearly basis
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Papers
Journal Article•
TL;DR: A 30 years old male patient presented with 5 years history of transfusion dependent anemia with intermittent episodes of passing dark colored urine in the morning, confirming the diagnosis of paroxysmal nocturnal hemoglobinuria.
Abstract: Paroxysmal Nocturnal Hemoglobinuria (PNH) literally means to have episodes of hemoglobin in the urine during the night. It is a Coomb's negative rare hemolytic disorder characterized by non-malignant clonal expansion of haemopoietic stem cells due to acquired genetic mutations. A 30 years old male patient presented with 5 years history of transfusion dependent anemia with intermittent episodes of passing dark colored urine in the morning. Blood complete picture showed decreased hemoglobin and reticulocytosis upto 30%. Coomb's test was negative with unconjugated hyperbilirubinemia and markedly raised serum LDH. Urine analysis showed marked hemosiderinuria and flow cytometry revealed 60% RBCs deficient for CD-59, confirming the diagnosis of paroxysmal nocturnal hemoglobinuria. The management of the patient depends on whether anemia is due to hemolysis or as consequence of impaired erythropoiesis. Corticosteroids at a dose of 0.25-1 mg/kg/day was selected as it is amongst the various treatment options in patients with predominant hemolysis.
316 citations
Journal Article•
TL;DR: The percentage of motile spermatozoa found to be coated with antisperm antibody of the IgG class, and the extent of the coating, proved to be correlated with the agglutination titer of circulating antisperm antibodies and with the inhibition of sperm penetration into cervical mucus.
Abstract: A simple and rapid test for the detection of antisperm antibodies of the IgG class on freely swimming spermatozoa in fresh human semen is described. The test is based on the formation of motile mixed agglutinates between erythrocytes sensitized with incomplete anti-Rh-antibodies and freely swimming spermatozoa with surface antisperm antibodies, after mixing both cell types together with anti-IgG antiserum. Agglutination of the red blood cells serves as an internal control. The test can be applied on ejaculates with spermatozoa concentrations down to one million per ml, provided the motility is sufficient. The percentage of motile spermatozoa found to be coated with antisperm antibodies of the IgG class, and the extent of the coating, proved to be correlated with the agglutination titer of circulating antisperm antibodies and with the inhibition of sperm penetration into cervical mucus. The test can be used as a screening for the presence of antisperm autoantibodies in serum and semen.
241 citations
TL;DR: During this investigation trypsin has been found to cause agglutination of cells sensitized with an ‘incomplete’ antibody, and also to enhance the specific agglUTination of other hæmagglutinins in the absence of any detectable antibody of the ‘ in complete’ type.
Abstract: In an attempt to define the properties of the enzyme present in the filtrate of a culture of vibrio cholera, which causes cells sensitized with an ‘incomplete’ anti-Rh antibody to agglutinate1, cultures of other organisms and pure enzyme preparations have been tested. During this investigation trypsin has been found to cause agglutination of cells sensitized with an ‘incomplete’ antibody, and also to enhance the specific agglutination of other haemagglutinins in the absence of any detectable antibody of the ‘incomplete’ type.
178 citations
TL;DR: This antibody is found only in donors who had previously received penicillin, and there is an increased incidence of the antibody in allergic persons.
Abstract: CIRCULATING antipenicillin antibody (CAPA) was first reported by Ley et al.1 During routine blood-banking procedures the serum of a prospective transfusion recipient was found to agglutinate an entire panel of erythrocytes that had been stored with penicillin as part of the preservative; erythrocytes from the same donors, when not exposed to penicillin, were not agglutinated by this serum. Ley and his co-workers1 and Watson, Joubert and Bennett,2 in tests on 2000 and 3000 unselected serums, respectively, found this antibody only in donors who had previously received penicillin. Although there is an increased incidence of the antibody in allergic persons3 4 5 6 7 8 it . . .
178 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 5 |
| 2024 | 16 |
| 2023 | 28 |
| 2022 | 37 |
| 2021 | 11 |
| 2020 | 16 |