Topic
Conopomorpha sinensis
About: Conopomorpha sinensis is a research topic. Over the lifetime, 58 publications have been published within this topic receiving 188 citations.
Papers published on a yearly basis
Papers
TL;DR: CsVg might be a better parameter than CsVGR for assessing the effect of sublethal insecticides on reproduction in C. sinensis, and EB elicits a delayed response in the abundance of C sVgR in response to the sublethal effect of insecticides.
Abstract: Conopomorpha sinensis Bradley is the dominant borer pest of Litchi chinesis and Euphoria longan. Current management of C. sinensis relies upon insecticide application to adult moths. In addition to the direct mortality induced by insecticides, a sublethal dose of insecticides also affects growth, survival, and reproduction in the exposed insects. Vitellogenin (Vg) and vitellogenin receptor (VgR) are normally identified as essential reproduction-related proteins in insects. In this study, we characterized these two genes from C. sinensis, and investigated their differential responses to sublethal concentrations of insecticide. Cloned CsVg and CsVgR consist of 5391 and 5424-bp open reading frames, which encode proteins of 1796 and 1807 amino acid residues, respectively. The CsVg protein contains the typical vitellogenin, DUF1943 and VWFD domains as other reported lepidopteran Vgs. The CsVgR was characterized as a typical low density lipoprotein receptor with two highly conserved LBD and EGF precursor domains, one hydrophobic transmembrane domain, one cytoplasmic domain, and 13 putative N-glycosylation sites. We next assessed the sublethal effect of four major insecticides on egg-laying in C. sinensis. The toxicity against C. sinensis varied among the insecticides tested, with LC50 values ranging from 0.23 ppm for chlorpyrifos to 20.00 ppm for β-cypermethrin, among which emamectin benzoate (EB) showed a significant negative impact on egg-laying, survival rate, ovarian development, and mating rate of C. sinensis at LC30 doses. Further investigation showed that the transcriptional level of CsVg and CsVgR were impaired in different way at 24, 48, and 72 h after EB exposure, and this result was in agreement with the diminished egg-laying of C. sinensis in the sublethal concentration EB-treated group. A repressed transcription level of CsVgR was observed at 48 h after treatment, suggesting that EB elicits a delayed response in the abundance of CsVgR. These results established different roles of CsVg and CsVgR in response to the sublethal effect of insecticides. CsVg might be a better parameter than CsVgR for assessing the effect of sublethal insecticides on reproduction in C. sinensis.
49 citations
TL;DR: The identity and scientific name of the cocoa moth or cocoa pod borer, Conopomorpha cramerella (Snellen), the major pest of cocoa in South-East Asia and generally recorded in the literature as Acrocercops cramella, are clarified.
Abstract: The identity and scientific name of the cocoa moth or cocoa pod borer, Conopomorpha cramerella (Snellen), the major pest of cocoa in South-East Asia and generally recorded in the literature as Acrocercops cramerella, are clarified. Three previously unrecognized congeneric species are described and differentiated, viz. C. oceanica sp. n., C. sinensis sp. n. and C. litchiella sp. n. Wing pattern, male and female genitalia are figured for each species.
28 citations
TL;DR: Functional difference between these two CsGOBPPs in perception of host plant odourants is strongly suggested by real-time PCR assays.
Abstract: BACKGROUND
The litchi fruit borer, Conopomorpha sinensis Bradley, is one of the most destructive pests of litchi and longan fruits in south-east Asia and southern China, yet the molecular biology and physiology of this pest remain poorly understood. Control of this insect pest may be achieved by interfering with its recognition of host plants.
RESULTS
In this study, two cDNAs encoding CsGOBP1 and CsGOBP2 were identified from the antennae of C. sinensis, and a comparative study on these two C. sinensis GOBPs (CsGOBPs) was conducted. The secondary structure of these two CsGOBPs mainly consists of six α-helices, but three-dimensional structural predictions of CsGOBP1 and CsGOBP2 indicated significant difference in the final 3D models. Results in real-time PCR assays indicated that the two CsGOBPs had different tissue- and sex-dependent expression patterns. A competitive binding assay revealed that CsGOBP1 considerably prefer the component exhibited in Guiwei or Feizixiao litchi cultivar, while CsGOBP2 bind to general volatile components from nine litchi cultivars. Additionally, ethyl acetate has higher binding affinities to CsGOBP2 protein than to CsGOBP1, and has remarkable attraction to female C. sinensis moths in Y-tube olfactometer assays.
CONCLUSION
These results strongly suggest functional difference between these two CsGOBPs in perception of host plant odourants. © 2015 Society of Chemical Industry
26 citations
TL;DR: It is demonstrated that C. sinensis has evolved special physical and chemical mechanisms for host plant location that enable it to survive and reproduce.
Abstract: Conopomorpha sinensis Bradley is a host-specific pest of Litchi chinensis and Euphoria longan. Here, we demonstrated that C. sinensis has evolved special physical and chemical mechanisms for host plant location that enable it to survive and reproduce. Females favored laying their eggs on the convex surface of litchi fruit that had particular volatile characteristics. Experiments using a H-type olfactometer showed that female C. sinensis were attracted to litchi flowers, tender shoots, immature fruits, and mature fruits, with the highest attraction rate to mature fruits (74.67 ± 2.31%). There were no significant differences in the attraction of male C. sinensis to different litchi tissues. Further oviposition preference tests using the pericarp, pulp, and seeds of mature litchi fruits revealed that female C. sinensis prefer to lay their eggs on the pericarp. Litchi volatiles were found to be important in attracting C. sinensis to fruits for oviposition. Analysis of volatiles from different litchi tissues by HS-SPME-GC-MS revealed 31 similar volatiles, some of which may be important in the oviposition preference choices of C. sinensis on litchi fruit.
19 citations
TL;DR: Phylogenetic analyses of D1/D2 26S rDNA and internal transcribed spacer (ITS) sequences indicate that the yeasts are closely related to Bullera dendrophila and an undescribed species of Cryptococcus (strain CBS 8507).
Abstract: Two new yeast species, Cryptococcus bestiolae and Cryptococcus dejecticola, were discovered in the frass of the litchi fruit borer Conopomorpha sinensis Bradley. The yeasts utilize inositol, hydrolyze urea, produce starch-like substance, and contain CoQ10. Phylogenetic analyses of D1/D2 26S rDNA and internal transcribed spacer (ITS) sequences indicate that the yeasts are closely related to Bullera dendrophila and an undescribed species of Cryptococcus (strain CBS 8507). The two new species differed from each other by 17 nucleotides in the D1/D2 region and by 68 nucleotides in the ITS region. Cryptococcus bestiolae is a sister species to Cryptococcus sp. CBS 8507, from which it differs by eight nucleotides in the D1/D2 region and 59 nucleotides in the ITS region. Cryptococcus dejecticola and B. dendrophila differed by 13 nucleotides in the D1/D2 and 57 nucleotides in the ITS region. Cryptococcus bestiolae and Cr. dejecticola formed with B. dendrophila a well defined clade consisting of insect associated species. The type strain of Cr. bestiolae is TH3.2.59 (=CBS 10118=NRRL Y-27894), and the type strain of Cr. dejecticola is Litch 17 (=CBS 10117=NRRL Y-27898).
19 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2020 | 2 |
| 2019 | 6 |
| 2018 | 5 |
| 2017 | 5 |
| 2016 | 4 |