TL;DR: A chemically defined medium that supports rapid and abundant growth of A. conoides is described and it is shown that the fungus is unable to synthesize either of the two moieties of thiamine but completes biosynthesis of the vitamin via a coupling reaction.
Abstract: Coscarelli, Waldimero (Rutgers, The State University, New Brunswick, N.J.) and David Pramer. Nutrition and growth of Arthrobotrys conoides. J. Bacteriol. 84:60-64. 1962-Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus Arthrobotrys conoides does not demonstrate a unique nutritional pattern. Yeast extract, required for growth in a glucose-inorganic salts medium, was replaced by biotin, thiamine, and zinc. Biotin biosynthesis by the fungus is blocked at the desthiobiotin to biotin conversion. A. conoides is unable to synthesize either of the two moieties of thiamine but, when supplied with pyrimidine and thiazole, completes biosynthesis of the vitamin via a coupling reaction. A chemically defined medium that supports rapid and abundant growth of A. conoides is described. Glucose is utilized efficiently (economic coefficient of 31.8 to 42.8), and yields exceeding 9 g (dry wt) of fungus tissue per liter of medium are obtained.
TL;DR: The trapping capability of Arthrobotrys oligospora and A. conoides against third stage larvae (L3) of Haemonchus contortus (Trichostrongylidae) was evaluated in an in vitro trial and the trapping capability shown was higher than 90% for both kinds of nematode.
Abstract: The trapping capability of Arthrobotrys oligospora and A. conoides (Hyphomycetales) against third stage larvae (L3) of Haemonchus contortus (Trichostrongylidae) was evaluated in an in vitro trial. Arthrobotrys oligospora showed a 35.87% and 25.71% trapping effectiveness against H. contortus infective larvae at 18 and 25°C, respectively; whereas the trapping capability of A. conoides was 92.17% and 90.40% at the same temperatures, respectively. Microscopic examination demonstrated that A. conoides spontaneously developed a large quantity of three-dimensional loops before the nematodes were added. Neither of the two species studied developed three-dimensional adhesive loops at 30°C, consequently no trapped nematode was observed. In a second trial, the trapping capability of A. conoides against H. contortus (L3) and second stage larvae (J2) of Nacobbus aberrans (Pratylenchidae), was evaluated at 25°C. The trapping capability shown by A. conoides was higher than 90% for both kinds of nematode. The possible use of A. conoides to control ovine haemonchosis is discussed.
TL;DR: Aqueous and ethanolic extracts from four species of seaweed: Sargassum binderi Sonder, Amphiroa sp., Turbinaria conoides (J. Agardh) Kuzting and Halimeda macroloba Decaisne from the east cost of the Gulf of Thailand were screened for antibacterial and antifungal activities as mentioned in this paper.
Abstract: Aqueous (AE) and ethanolic extracts (EE) from four species of seaweed: Sargassum binderi Sonder, Amphiroa sp., Turbinaria conoides (J. Agardh) Kuzting and Halimeda macroloba Decaisne from the east cost of the Gulf of Thailand were screened for antibacterial and antifungal activities. The test organisms were Staphylococcus aureus, S. epidermidis, Propionibacterium acnes, Proteus mirabilis and Candida albicans. Extracts of all the species were found to have antimicrobial activities. AE of T. conoides, however demonstrated the maximum efficacy. Therefore, AE of T. conoides was also evaluated for its anti-inflammatory effect using EPP-induced ear edema and carrageenin-induced hind paw edema tests. Results revealed anti-inflammatory activity of AE of T. conoides comparable to that phenylebutazol and acetylsalicylic acid used as standard controls.
TL;DR: The results cast doubt upon the validity of using intramarginal teeth for species diagnosis of Turbinaria ornata and T. conoides, two morphologically similar brown algae.
TL;DR: Evidence is provided that nemin-induced morphogenesis in A. conoides is dependent on carbon dioxide, a nematode-trapping fungus which captures prey in adhesive networks of hyphal loops.
Abstract: CARBON dioxide has been shown to influence morphogenesis in micro-organisms, higher plants and animals. Among the processes affected by carbon dioxide (or bicarbonate) concentration are sporangial differentiation in Blastocladiella emersonii1, mould-yeast dimorphism2,3, spherulation in Coccidioides immitis4, sporulation by bacteria5 and fungi6,7, root development by pea8, and sexual differentiation in Hydra9. This list can now be extended to include morphogenesis in Arthrobotrys conoides. A. conoides is a nematode-trapping fungus which captures prey in adhesive networks of hyphal loops. Usually, these organelles of capture are not formed spontaneously by the fungus but develop in response to the presence of prey. Morphogenesis is induced by a metabolic product of nematodes which has been partially purified10–12 and designated nemin13. The tests described here provide evidence that nemin-induced morphogenesis in A. conoides is dependent on carbon dioxide.