TL;DR: In this article, the biochemical composition of two Marine eels (Thyrsoidea macrura and Congresox talabanoides) from Annankovil landing centre southeast coast of India was elucidated.
Abstract: The aim of this present study was to elucidate the biochemical composition of two Marine eels (Thyrsoidea macrura and Congresox talabanoides) from Annankovil landing centre southeast coast of India. The proximate analysis exposed that the protein content of T. macrura and C. talabanoides was 22.7%, 25.8%, respectively, the total lipid content was high, ranging from 5.8% and 7.9%, the crude ash ranged from 6.9% and 7.1%. The major amino acids were glutamic acid, histidine and glycine ranging from (0.28 and 4.7%). The other dominant fatty acids detected were C18:1, C18:3, C20:5, and C22:6 ranging from 0.00212 and 24.12% in T. macrura and C. talabanoides. The level of Arachidonic Acid (AA) in Conger eel was 2.31% and Moray eel was about 2.01. The high levels of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) content of Moray and Conger eel makes its fatty acid profile more favorable.
TL;DR: Growth of test bacteria Escherichia coli ATCC 8739 and Staphylococcus aereus 6538 showed peptone yellow pike conger entrails can be used as a nutrient growth of bacteria on the growth medium.
Abstract: The research was conducted in March-April 2015 in the Laboratory of Fish Processing Technology, Laboratory of Microbiology and Biotechnology, Faculty of Fisheries and Marine Sciences, University of Riau, Laboratory of Analytical Chemistry, Faculty of Mathematics and Science, University of Riau and the Laboratory of Chemical Physics, Faculty of Mathematics and Science, University of North Sumatra. This study aims to produce peptone from waste yellow pike conger entrails as bacterial nutrients to support the growth of bacteria in the growth medium. Waste yellow pike conger entrails autolysis hydrolysed by activating proteolytic enzymes (pepsin) in the stomach of fish using 12 N HCl at pH (1,2,3 and 4) the hydrolysis temperature 40oC for 24 hours to determine the optimum hydrolysis conditions. The optimum hydrolysis conditions of waste yellow pike conger entrails occurs at pH 3, the temperature of 40oC hydrolysis with hydrolysis time of 24 hours, the solution was centrifuged extract peptone hydrolysis and then dried with a freeze dryer. The results showed peptone fish entrails have cunang content of 79.06% protein content; total nitrogen 12.66%; free α-amino nitrogen 1.97%; the ratio AN / TN 15.56%; water content of 5.43% and 93.03% solubility in water. Growth of test bacteria Escherichia coli ATCC 8739 and Staphylococcus aereus 6538 showed peptone yellow pike conger entrails can be used as a nutrient growth of bacteria on the growth medium.Keywords : Peptone, yellow pike conger, autolysis, enzyme pepsin, E. coli ATCC 8739, S. aereus 6538