Topic
Concatemer
About: Concatemer is a research topic. Over the lifetime, 464 publications have been published within this topic receiving 18358 citations.
Papers published on a yearly basis
1 / 2
Papers
TL;DR: The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails as discussed by the authors, and they can then join to form dimers which on further replication similarly form larger concatures.
Abstract: The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails. Right-ended and left-ended molecules can then join to form dimers which on further replication similarly form larger concatemers.
1,579 citations
TL;DR: Double-stranded DNA fragments terminated at their 5′-ends by the singlestranded sequence pA-A-T-T-, generated by digestion of DNA with Eco RI restriction endonuclease, were ligated with Escherichia coli polynucleotide ligase under various conditions of temperature, concentration and time.
434 citations
TL;DR: Polynucleotide ligase has been purified 1000-fold from extracts of Escherichia coli infected with bacteriophage T4 to eliminate the necessity for enzyme assays during the early fractionation steps and to permit the use of a simple assay, based on ATP-PPi exchange, during the later stages of purification.
394 citations
TL;DR: It is concluded that an intact prohead structure is required to mature the virus DNA (i.e. to cut the overlength DNA concatemer to the mature length) and apparently this cutting occurs as part of the encapsulation event.
301 citations
TL;DR: A tightly controlled process for strand-specific amplification of circularized DNA molecules that is suitable for parallel amplification of large numbers of DNA circles, because the few cycles and the robust reaction mechanism preserves the proportion of amplified molecules.
Abstract: We present a tightly controlled process for strand-specific amplification of circularized DNA molecules. Tandem repeated complements of DNA circles are generated by rolling-circle replication, and converted to monomer circles of opposite polarity to that of the starting material. These circles are then subjected to one more round of rolling-circle replication and circularization, and the process can be further repeated. The method can be directed to produce single-stranded circular or linear monomers, or linear concatemers of the desired polarity. The reaction is not product inhibited, and can yield ≈100-fold higher concentrations of monomer products than PCR. Each generation of the amplification process proceeds in a linear fashion, ensuring precise quantification. The procedure is suitable for parallel amplification of large numbers of DNA circles, because the few cycles and the robust reaction mechanism preserves the proportion of amplified molecules. We demonstrate the utility of the method for multiplexed genotyping of polymorphic loci and for quantitative DNA analysis.
272 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 4 |
| 2024 | 9 |
| 2023 | 34 |
| 2022 | 16 |
| 2021 | 14 |
| 2020 | 14 |